Immunohistochemical localization of SNARE core proteins in intrapulpal and intradentinal nerve fibers of rat molar teeth
| Autor: | Akiyo Kawano, Kohki Kadono, Shiho Honma, Satoshi Wakisaka |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Nerve Tissue Proteins Synaptic vesicle Exocytosis Gene product Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Nerve Fibers medicine Animals Receptor Microscopy Immunoelectron General Dentistry Dental Pulp Odontoblasts Chemistry Nerve plexus Cell Biology General Medicine Syntaxin 1 Fusion protein Immunohistochemistry Molar Axons Cell biology Rats 030104 developmental biology Dentinal Tubule medicine.anatomical_structure Otorhinolaryngology Dentin Synapses Dental Pulp Cavity SNARE Proteins Ubiquitin Thiolesterase 030217 neurology & neurosurgery |
| Zdroj: | Archives of oral biology. 73 |
| ISSN: | 1879-1506 |
| Popis: | Objective The present study was designed to elucidate whether three soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) core proteins, syntaxin-1, synaptosomal-associated protein of 25 kDa (SNAP-25), and vesicle-associated membrane protein-2 (VAMP-2), are present in the dental pulp of the rat molar at both the light and electron microscopic levels. Design Immunohistochemistry for protein gene product 9.5 (PGP 9.5), a pan-neuronal marker, syntaxin-1, SNAP-25, and VAMP-2 was performed on decalcified rat molars for light and electron microscopic analyses. Double-immunolabeling of PGP 9.5 and the SNARE core proteins, as well as combinations of the SNARE core proteins, was also carried out. Results PGP 9.5-immunoreactive nerve fibers ran toward the coronal region, ramified at the subodontoblast layer, and formed the subodontoblastic nerve plexus. Most nerve fibers penetrated the predentin and dentin along the dentinal tubules. Most, if not all, nerve fibers displayed immunoreactivity for syntaxin-1, SNAP-25, and VAMP-2. Immunoelectron microscopic analyses confirmed the presence of immunoreactivity for the SNARE core proteins within the intradental axonal elements. Conclusions The present findings suggest that, since SNARE core proteins participate in the docking and exocytosis of synaptic vesicles in the central nervous system, they may contribute to vesicle exocytosis from the dental nerve fibers even though there are no apparent synapses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect