Identification of Single-Base Mismatches in Pneumocystis jirovecii Isolated from Iranian TB positive Patients by CSGE Heteroduplex
| Autor: | Mehdi Razzaghi Abyaneh, Masoomeh Shams Ghahfarokhi, Behnam Ghalehbin, Saeideh Ghayoum |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology business.industry 030106 microbiology dihydropteroate synthase (dhps) biology.organism_classification Applied Microbiology and Biotechnology Microbiology Virology QR1-502 pneumocystis jirovecii 03 medical and health sciences parasitic diseases heteroduplex csge lactate dehydrogenase (ldh) Pneumocystis jirovecii Medicine Identification (biology) business Base (exponentiation) gene mutations Biotechnology Heteroduplex |
| Zdroj: | Journal of Pure and Applied Microbiology, Vol 11, Iss 3, Pp 1287-1292 (2017) |
| ISSN: | 0973-7510 |
| DOI: | 10.22207/jpam.11.3.08 |
| Popis: | Pneumocystis jirovecii is an opportunistic fungus, which causes Pneumocystis pneumonia (PJP) in immunocompromised, COPD and TB positive patients with a high rate of colonization, morbidity and mortality. Dihydropteroate synthase (DHPS) gene mutations are well-reported in PJP. Although sulfa prophylaxis generally is associated with DHPS mutant infection, Multiple molecular techniques applied for detect sulfa resistance single-base mutation. Conformation sensitive gel electrophoresis (CSGE) is a rapid screening method for detection of DNA sequence variation, specifically single-base changes or small insertions and deletions. The current study is investigate on the DHPS sequence single-base dislocation among strains isolated from Iranian TB positive co-infected with PJP in association to increased levels of serum Lactate Dehydrogenase. Through high serum lactate dehydrogenase (LDH) levels have been associated with established Pneumocystis pneumonia. We investigated the DHPS mismatches in five P. jirovecii isolated of TB infected patients. For genetic identification of Pneumocystis isolates and detection of intraspecific variation, we developed a method for heteroduplex analysis. Our utilizing fragments was the DHPS gene regions, amplified by PCR method with specific primers. Serum LDH indicator was analysed for lung acute damages. In our results, at least 4 suspected isolates show more slowly migrating bands containing single heteroduplexes, reveal single-base mislocation in studied sequences. LDH level Peak was higher (p |
| Databáze: | OpenAIRE |
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