Development of novel guanidino-labelling derivatisation (GLaD) reagents for liquid chromatography/matrix-assisted laser desorption/ionisation analysis
| Autor: | Francesco L. Brancia, Daniela Delneri, Jessica Z. Bereszczak, Elzbieta Piatkowska |
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| Rok vydání: | 2007 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Chromatography Staining and Labeling Chemistry Organic Chemistry Quantitative proteomics Proteins Reproducibility of Results Mass spectrometry Peptide Mapping Sensitivity and Specificity Analytical Chemistry Isotopomers Ion Matrix (chemical analysis) Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Reagent Ionization Desorption Indicators and Reagents Chromatography High Pressure Liquid Guanidine Spectroscopy |
| Zdroj: | Rapid Communications in Mass Spectrometry. 21:3069-3076 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/rcm.3176 |
| Popis: | A new generation of guanidino-labelling (GLaD) reagents were developed for quantitative proteomics using offline microcapillary liquid chromatography (LC) matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS). In order to reduce the unwanted overlapping between the isotopic envelopes of the two differentially labelled peptide ions, a novel synthetic route was described for production of both (13)C- and (15)N-containing isotopomers of N,O-dimethylisourea. The use of these types of isotopes has no deleterious effect on the retention times of both differentially labelled peptides during offline microbore reversed-phase LC. In addition, the possibility to incorporate a mass difference of 4 Da can be exploited during post-source decay analysis to generate product ion spectra in which fragment ions containing the modifications appear as doublets in the corresponding product ion spectra, thus facilitating identification of the C-terminal fragment ions. |
| Databáze: | OpenAIRE |
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