First ovarian response to gonadotrophin stimulation in rats exposed to neonatal androgen excess
Autor: | Gabriel Anesetti, Rebeca Chávez-Genaro |
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Rok vydání: | 2018 |
Předmět: |
Ovulation
0301 basic medicine endocrine system medicine.medical_specialty Histology Physiology media_common.quotation_subject Ovary Stimulation Chorionic Gonadotropin 03 medical and health sciences Follicle 0302 clinical medicine Ovarian Follicle Internal medicine Follicular phase medicine Animals Testosterone media_common 030219 obstetrics & reproductive medicine business.industry Dihydrotestosterone Cell Biology General Medicine Neonatal androgen Rats 030104 developmental biology medicine.anatomical_structure Endocrinology Animals Newborn Androgens Female business Gonadotropins hormones hormone substitutes and hormone antagonists medicine.drug |
Zdroj: | Journal of Molecular Histology. 49:631-637 |
ISSN: | 1567-2387 1567-2379 |
Popis: | This study analyzes the effects of neonatal androgenization on follicular growth and first ovulation in response to gonadotrophins, using a model of exogenous stimulation or the use of subcutaneous ovary grafts in castrated animals to replace the hypothalamus-pituitary signal. Neonatal rats (days 1-5) were treated with testosterone, dihydrotestosterone or vehicle. At juvenile period, rats were stimulated with PMSG, hCG (alone or combined) or used as ovarian donors to be grafted on castrated adult female rats. Ovulation and ovarian histology were analyzed in both groups. Animals treated with vehicle or dihydrotestosterone stimulated with gonadotrophins (pharmacological or by using an ovary graft) ovulated, showing a normal histological morphology whereas rats exposed to testosterone and injected with the same doses of gonadotrophins did not it. In this group, ovulation was reached using a higher dose of hCG. Ovaries in the testosterone group were characterized by the presence of follicles with atretic appearance and a larger size than those observed in control or dihydrotestosterone groups. A similar appearance was observed in testosterone ovary grafts although luteinization and some corpora lutea were also identified. Our findings suggest that neonatal exposure to aromatizable androgens induces a more drastic signalling on the ovarian tissue that those driven by non-aromatizable androgens in response to gonadotrophins. |
Databáze: | OpenAIRE |
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