Nature and regulation of pistil-expressed genes in tomato
| Autor: | Stephen B. Milligan, Charles S. Gasser |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Gynoecium
DNA Complementary Time Factors Molecular Sequence Data Plant Science Biology Genes Plant Sepal Leucyl Aminopeptidase Cellulase Solanum lycopersicum Gene Expression Regulation Plant Complementary DNA Genetics Genomic library Tissue Distribution Amino Acid Sequence RNA Messenger Peptide sequence Leucyl aminopeptidase Gene Gene Library Glycoproteins Plant Proteins Sequence Homology Amino Acid Reproduction food and beverages Gene Expression Regulation Developmental General Medicine Sequence Analysis DNA Blotting Northern Petal Agronomy and Crop Science Antimicrobial Cationic Peptides |
| Zdroj: | Plant molecular biology. 28(4) |
| ISSN: | 0167-4412 |
| Popis: | The specialized reproductive functions of angiosperm pistils are dependent in part upon the regulated activation of numerous genes expressed predominantly in this organ system. To better understand the nature of these pistil-predominant gene products we have analyzed seven cDNA clones isolated from tomato pistils through differential hybridization screening. Six of the seven cDNAs represent sequences previously undescribed in tomato, each having a unique pistil- and/or floral-predominant expression pattern. The putative protein products encoded by six of the cDNAs have been identified by their similarity to sequences in the database of previously sequenced genes, with a seventh sequence having no significant similarity with any previously reported sequence. Three of the putative proteins appear to be targeted to the endomembrane system and include an endo-beta-1,4-glucanase which is expressed exclusively in pistils at early stages of development, and proteins similar in sequence to gamma-thionin and miraculin which are expressed in immature pistils and stamens, and in either sepals or petals, respectively. Two other clones, similar in sequence to each other, were expressed primarily in immature pistils and stamens and encode distinct proteins with similarity to leucine aminopeptidases. An additional clone, which encodes a protein similar in sequence to the enzyme hyoscyamine 6-beta-hydroxylase and to other members of the family of Fe2+/ascorbate-dependent oxidases, was expressed at high levels in pistils, stamens and sepals, and at detectable levels in some vegetative organs. Together, these observations provide new insight into the nature and possible functional roles of genes expressed during reproductive development. |
| Databáze: | OpenAIRE |
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