Use of molecular markers and flow cytometry to preserve ancient Annurca apple germplasm
| Autor: | Rosanna Capparelli, Daniela Palumbo, Ilaria Ventimiglia, Marco Iannaccone, Paola Spigno, Andrea Patocchi |
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| Přispěvatelé: | Iannaccone, Marco, Palumbo, D, Ventimiglia, I, Patocchi, A, Spigno, P, Capparelli, Rosanna |
| Předmět: |
Germplasm
Malus DNA Plant Bioengineering Biology Applied Microbiology and Biotechnology chemistry.chemical_compound Molecular marker Botany Genetic variability Cultivar DNA Primers Electrophoresis Agar Gel fungi Genetic Variation food and beverages Annurca apple - Flow cytometry - Germplasm management - Molecular markers General Medicine Flow Cytometry biology.organism_classification Random Amplified Polymorphic DNA Technique RAPD chemistry Fruit Field management Microsatellite Repeats Biotechnology |
| Zdroj: | Europe PubMed Central |
| Popis: | The old Annurca apple cultivar (Malus domestica), particularly appreciated for its peculiar flavor and crispy flesh, was studied in order to preserve its ancient germplasm. Twelve clones of Annurca were analyzed using random amplified polymorphic DNA (RAPD) and simple-sequence repeat (SSR) markers. Two out of 30 RAPD primers and nine out of ten SSR primers were able to discriminate all the clones analyzed. Data were confirmed by measuring DNA content using flow cytometry. The results provide a good procedure to improve germplasm field management, in order to removing redundant material in the Annurca collection. This represents an efficient way to create a data bank in order to preserve the genetic variability of the Annurca cultivar. |
| Databáze: | OpenAIRE |
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