A minimum number of autoimmune T cells to induce autoimmunity?
| Autor: | Aleksandra J. Ozga, Jens V. Stein, Beatrice Bolinger, Virginie Galati-Fournier, Ed Palmer, Simone Keck, Angela J.T. Bosch, Ondrej Stepanek |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ovalbumin T cell Immunology Priming (immunology) Autoimmunity Mice Transgenic Biology CD8-Positive T-Lymphocytes Immune tolerance Autoimmune Diseases Diabetes Mellitus Experimental 03 medical and health sciences Interleukin 21 Mice 0302 clinical medicine medicine Immune Tolerance Cytotoxic T cell Animals IL-2 receptor 610 Medicine & health Autoimmune disease Antigen Presentation Dendritic Cells medicine.disease Adoptive Transfer Disease Models Animal 030104 developmental biology medicine.anatomical_structure CTLA-4 Lymph Nodes 030215 immunology |
| Zdroj: | Cell. Immunol. |
| Popis: | While autoimmune T cells are present in most individuals, only a minority of the population suffers from an autoimmune disease. To better appreciate the limits of T cell tolerance, we carried out experiments to determine how many autoimmune T cells are required to initiate an experimental autoimmune disease. Variable numbers of autoimmune OT-I T cells were transferred into RIP-OVA mice, which were injected with antigen-loaded DCs in a single footpad; this restricted T cell priming to a few OT-I T cells that are present in the draining popliteal lymph node. Using selective plane illumination microscopy (SPIM) we counted the number of OT-I T cells present in the popliteal lymph node at the time of priming. Analysis of our data suggests that a single autoimmune T cell cannot induce an experimental autoimmune disease, but a "quorum" of 2-5 autoimmune T cells clearly has this capacity. |
| Databáze: | OpenAIRE |
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