Quality and intensity of the tissue response to two synthetic granular hydroxyapatite implanted in critical defects of rat calvaria
| Autor: | Neusa Motta de Freitas Costa, Gustavo Vicentis de Oliveira Fernandes, Gloria Dulce de Almeida Soares, José Mauro Granjeiro, Rosana Tavares Brito, Eliene Carvalho da Fonseca, Márcio Baltazar Conz, Bruno Raposo Melo, Vagner Gonçalves Bernardo |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Materials science Cell growth Angiogenesis Mechanical Engineering Microvascular Density hydroxyapatite Calvaria Bone healing Condensed Matter Physics bone repair histomorphometry Crystallinity medicine.anatomical_structure Mechanics of Materials Giant cell immunohistochemistry medicine lcsh:TA401-492 Immunohistochemistry General Materials Science lcsh:Materials of engineering and construction. Mechanics of materials Biomedical engineering |
| Zdroj: | Materials Research, Vol 12, Iss 2, Pp 245-251 (2009) Materials Research v.12 n.2 2009 Materials research (São Carlos. Online) Universidade Federal de São Carlos (UFSCAR) instacron:ABM ABC ABPOL Materials Research, Volume: 12, Issue: 2, Pages: 245-251, Published: JUN 2009 |
| ISSN: | 1516-1439 |
| Popis: | The objective of the present study was to evaluate the quality and intensity of the tissue response to two synthetic hydroxyapatites implanted in critical defects in the skulls of rats. Sixty animals were divided into three experimental groups: I (control), II (HA-1 = HA with 28% crystallinity) and III (HA-2 = HA with 70% crystallinity). They were sacrificed 1, 3, 6, and 9 months after implantation (n = 5 individuals per group/period). Histomorphometric analysis included i) counting of polymorphonuclear leucocytes, mast cells, macrophages and foreign body multinucleated giant cells stained with anti-lysozyme; ii) microvascular density stained with anti-Factor VIII and iii) degree of cell proliferation stained with anti-PCNA. There were no significant differences between the experimental groups in either the quality or quantity of cells in the inflammatory infiltrate, or the degree of angiogenesis and cell proliferation. We conclude that HA-1 and HA-2 are biocompatible and that the physico-chemical differences of these biomaterials did not affect cellular response. |
| Databáze: | OpenAIRE |
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