Odorant-Dependent Generation of Nitric Oxide in Mammalian Olfactory Sensory Neurons

Autor: Stefan Kurtenbach, Daniela Brunert, Christian H. Wetzel, Günter Gisselmann, Wolfgang Schuhmann, Sonnur Isik, Hanns Hatt, Heike Benecke
Rok vydání: 2009
Předmět:
Olfactory system
Nitric Oxide Synthase Type III
Sensory Receptor Cells
Acyclic Monoterpenes
Green Fluorescent Proteins
lcsh:Medicine
Sensory system
Biology
Nitric Oxide
Epithelium
Cell Biology/Cell Signaling
Mice
Neuroscience/Neuronal Signaling Mechanisms
medicine
Animals
Enzyme Inhibitors
Olfactory memory
lcsh:Science
In Situ Hybridization
Mice
Knockout
Aldehydes
Physiology/Sensory Systems
Multidisciplinary
Physiology/Neuronal Signaling Mechanisms
Reverse Transcriptase Polymerase Chain Reaction
Terpenes
Neuroscience/Sensory Systems
lcsh:R
Colforsin
Neurogenesis
Immunohistochemistry
Olfactory Bulb
Olfactory bulb
Cell biology
Electrophysiology
Mice
Inbred C57BL
Nitric oxide synthase
NG-Nitroarginine Methyl Ester
medicine.anatomical_structure
Biochemistry
biology.protein
lcsh:Q
Calcium
Nitric Oxide Synthase
Signal transduction
Olfactory epithelium
Research Article
Zdroj: PLoS ONE
PLoS ONE, Vol 4, Iss 5, p e5499 (2009)
ISSN: 1932-6203
Popis: The gaseous signalling molecule nitric oxide (NO) is involved in various physiological processes including regulation of blood pressure, immunocytotoxicity and neurotransmission. In the mammalian olfactory bulb (OB), NO plays a role in the formation of olfactory memory evoked by pheromones as well as conventional odorants. While NO generated by the neuronal isoform of NO synthase (nNOS) regulates neurogenesis in the olfactory epithelium, NO has not been implicated in olfactory signal transduction. We now show the expression and function of the endothelial isoform of NO synthase (eNOS) in mature olfactory sensory neurons (OSNs) of adult mice. Using NO-sensitive micro electrodes, we show that stimulation liberates NO from isolated wild-type OSNs, but not from OSNs of eNOS deficient mice. Integrated electrophysiological recordings (electro-olfactograms or EOGs) from the olfactory epithelium of these mice show that NO plays a significant role in modulating adaptation. Evidence for the presence of eNOS in mature mammalian OSNs and its involvement in odorant adaptation implicates NO as an important new element involved in olfactory signal transduction. As a diffusible messenger, NO could also have additional functions related to cross adaptation, regeneration, and maintenance of MOE homeostasis.
Databáze: OpenAIRE
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