Odorant-Dependent Generation of Nitric Oxide in Mammalian Olfactory Sensory Neurons
Autor: | Stefan Kurtenbach, Daniela Brunert, Christian H. Wetzel, Günter Gisselmann, Wolfgang Schuhmann, Sonnur Isik, Hanns Hatt, Heike Benecke |
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Rok vydání: | 2009 |
Předmět: |
Olfactory system
Nitric Oxide Synthase Type III Sensory Receptor Cells Acyclic Monoterpenes Green Fluorescent Proteins lcsh:Medicine Sensory system Biology Nitric Oxide Epithelium Cell Biology/Cell Signaling Mice Neuroscience/Neuronal Signaling Mechanisms medicine Animals Enzyme Inhibitors Olfactory memory lcsh:Science In Situ Hybridization Mice Knockout Aldehydes Physiology/Sensory Systems Multidisciplinary Physiology/Neuronal Signaling Mechanisms Reverse Transcriptase Polymerase Chain Reaction Terpenes Neuroscience/Sensory Systems lcsh:R Colforsin Neurogenesis Immunohistochemistry Olfactory Bulb Olfactory bulb Cell biology Electrophysiology Mice Inbred C57BL Nitric oxide synthase NG-Nitroarginine Methyl Ester medicine.anatomical_structure Biochemistry biology.protein lcsh:Q Calcium Nitric Oxide Synthase Signal transduction Olfactory epithelium Research Article |
Zdroj: | PLoS ONE PLoS ONE, Vol 4, Iss 5, p e5499 (2009) |
ISSN: | 1932-6203 |
Popis: | The gaseous signalling molecule nitric oxide (NO) is involved in various physiological processes including regulation of blood pressure, immunocytotoxicity and neurotransmission. In the mammalian olfactory bulb (OB), NO plays a role in the formation of olfactory memory evoked by pheromones as well as conventional odorants. While NO generated by the neuronal isoform of NO synthase (nNOS) regulates neurogenesis in the olfactory epithelium, NO has not been implicated in olfactory signal transduction. We now show the expression and function of the endothelial isoform of NO synthase (eNOS) in mature olfactory sensory neurons (OSNs) of adult mice. Using NO-sensitive micro electrodes, we show that stimulation liberates NO from isolated wild-type OSNs, but not from OSNs of eNOS deficient mice. Integrated electrophysiological recordings (electro-olfactograms or EOGs) from the olfactory epithelium of these mice show that NO plays a significant role in modulating adaptation. Evidence for the presence of eNOS in mature mammalian OSNs and its involvement in odorant adaptation implicates NO as an important new element involved in olfactory signal transduction. As a diffusible messenger, NO could also have additional functions related to cross adaptation, regeneration, and maintenance of MOE homeostasis. |
Databáze: | OpenAIRE |
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