Neuronal properties and trophic activities of immortalized hippocampal cells from embryonic and young adult mice
| Autor: | Sim Ja, Lee Hj, Wainer Bh, Brown Da, Otten Uh, Hammond Dn, John D. Roback, Large Th |
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| Rok vydání: | 1990 |
| Předmět: |
Retinoic acid
Hippocampus Nerve Tissue Proteins Biology Hippocampal formation Hybrid Cells Cell Line chemistry.chemical_compound Mice Intermediate Filament Proteins Neurofilament Proteins Animals Nerve Growth Factors Neurons General Neuroscience Brain Articles Embryonic stem cell Choline acetyltransferase Electrophysiology Nerve growth factor chemistry Cell culture Cholinergic Neuroscience |
| Zdroj: | The Journal of neuroscience : the official journal of the Society for Neuroscience. 10(6) |
| ISSN: | 0270-6474 |
| Popis: | The hippocampal formation elaborates trophic factors such as nerve growth factor (NGF) to support the cholinergic innervation it receives from the septal region. To further study the trophic interactions of this pathway, hippocampal cells from embryonic day 18 and postnatal day 21 mice were immortalized via somatic cell fusion to N18TG2 neuroblastoma cells. The hippocampal cell lines exhibit morphological and cytoskeletal features which are typical of their neuronal parents but which are not expressed by the neuroblastoma parent. When differentiated with retinoic acid, the hippocampal cell lines exhibit electrophysiological features similar to cultured hippocampal neurons. Many of the lines constitutively express high levels of NGF, and at least one cell line exerts a non-NGF trophic effect on the expression of choline acetyltransferase by septal neurons in vitro. These cell lines are potentially useful for investigating the neurochemical and excitable properties of hippocampal neurons and identifying novel trophic activities that promote the development and maintenance of the septohippocampal pathway. |
| Databáze: | OpenAIRE |
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