Differential expression of porcine testis proteins during postnatal development
Autor: | Chin kai Chuang, En Chung Lin, Meng-Ting Chung, San-Yuan Huang, Jun Yi Liao, Hsin Hsin Chen, H. Sunny Sun, Yu-Hui Chen, Jyh Hung Lin, Mu-Chiou Huang, Shih-Hua Teng, Ming Yu Chen |
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Rok vydání: | 2010 |
Předmět: |
Male
Proteome Sequence analysis Sus scrofa Mitochondrion Biology Mass Spectrometry Andrology Endocrinology Food Animals Sequence Analysis Protein Organelle Testis Animals Cluster Analysis Electrophoresis Gel Two-Dimensional Testosterone Proteins General Medicine Molecular biology Immunohistochemistry Animals Newborn Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Metabolome Animal Science and Zoology Spermatogenesis Developmental biology Binding domain |
Zdroj: | Animal reproduction science. 123(3-4) |
ISSN: | 1873-2232 |
Popis: | The development of the testes includes changes in cell morphology and endocrine levels that are essential for the maturation of males. A large number of novel proteins are expressed throughout testis development and play important roles in spermatogenesis. Differences in protein expressions during the development of porcine testes have not been systematically studied. The purpose of this study was to investigate differential protein expression in porcine testes during postnatal development. Testes from four pigs each at 1wk, 3mo, and 1yr of age were used for a proteomic analysis. Expression levels of 264 protein spots were quantified using the Melanie 3 software. In total, 108 protein spots showed more than 2-fold differences (P |
Databáze: | OpenAIRE |
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