Post-Death Cloning of Endangered Jeju Black Cattle (Korean Native Cattle): Fertility and Serum Chemistry in a Cloned Bull and Cow and Their Offspring
| Autor: | Dong Hwan Song, Younghoon Kim, Chang Eon Oh, Seong Ho Mun, Eun Young Kim, Jeremiah Jiman Moon, Hyun Yong Choi, Seung-Eun Lee, Moon Suck Ko, Hyo Young Park, Key Zung Riu, Dong-Sun Lee, Se Pill Park, Min Jee Park |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Nuclear Transfer Techniques Cloning Organism medicine.medical_treatment Population Oocyte Retrieval Jeju Black Fertilization in Vitro Extinction Biological Insemination Embryo Culture Techniques Andrology Offspring Pregnancy Republic of Korea medicine Animals Post-death cloning education Cells Cultured Insemination Artificial reproductive and urinary physiology Sperm motility Genetics education.field_of_study biology urogenital system Artificial insemination Endangered Species SCNT Ear Embryo biology.organism_classification Sperm In Vitro Oocyte Maturation Techniques Fertility Blood chemistry Sperm Motility Cattle Ectogenesis Female Original Article Animal Science and Zoology Live Birth Jeju black cattle |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.2013-047 |
| Popis: | To preserve Jeju black cattle (JBC; endangered native Korean cattle), a pair of cattle, namely a post-death cloned JBC bull and cow, were produced by somatic cell nuclear transfer (SCNT) in a previous study. In the present study, we examined the in vitro fertilization and reproductive potentials of these post-death cloned animals. Sperm motility, in vitro fertilization and developmental capacity were examined in a post-death cloned bull (Heuk Oll Dolee) and an extinct nuclear donor bull (BK94-13). We assessed reproductive ability in another post-death cloned cow (Heuk Woo Sunee) using cloned sperm for artificial insemination (AI). There were no differences in sperm motility or developmental potential of in vitro fertilized embryos between the post-death cloned bull and its extinct nuclear donor bull; however, the embryo development ratio was slightly higher in the cloned sperm group than in the nuclear donor sperm group. After one attempt at AI, the post-death cloned JBC cow became pregnant, and gestation proceeded normally until day 287. From this post-death cloned sire and dam, a JBC male calf (Heuk Woo Dolee) was delivered naturally (weight, 25 kg). The genetic paternity/maternity of the cloned JBC bull and cow with regard to their offspring was confirmed using International Society for Animal Genetics standard microsatellite markers. Presently, Heuk Woo Dolee is 5 months of age and growing normally. In addition, there were no significant differences in blood chemistry among the post-death cloned JBC bull, the cow, their offspring and cattle bred by AI. This is the first report showing that a pair of cattle, namely, a post-death cloned JBC bull and cow, had normal fertility. Therefore, SCNT can be used effectively to increase the population of endangered JBC. |
| Databáze: | OpenAIRE |
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