Frequent interruptions of sedentary time modulates contraction- and insulin-stimulated glucose uptake pathways in muscle: Ancillary analysis from randomized clinical trials
| Autor: | David W. Dunstan, Alaina K. Natoli, Celine Latouche, Neville Owen, Bronwyn A. Kingwell, Audrey Bergouignan, Sarah E Heywood, Medini Reddy-Luthmoodoo, Megan S. Grace |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Blood Glucose medicine.medical_specialty Glucose uptake medicine.medical_treatment 030209 endocrinology & metabolism Carbohydrate metabolism Sitting Article Oxidative Phosphorylation 03 medical and health sciences 0302 clinical medicine Internal medicine Medicine Humans Insulin Phosphorylation Glycogen synthase Muscle Skeletal Multidisciplinary biology business.industry GTPase-Activating Proteins Glucose transporter Middle Aged Postprandial Period 030104 developmental biology Endocrinology Postprandial Glucose biology.protein Sedentary Behavior business Proto-Oncogene Proteins c-akt GLUT4 Acetyl-CoA Carboxylase Muscle Contraction |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Epidemiological studies have observed associations between frequent interruptions of sitting time with physical activity bouts and beneficial metabolic outcomes, even in individuals who regularly exercise. Frequent interruptions to prolonged sitting reduce postprandial plasma glucose. Here we studied potential skeletal muscle mechanisms accounting for this improved control of glycemia in overweight adults under conditions of one day uninterrupted sitting and sitting interrupted with light-intensity or moderate-intensity walking every 20-min (n = 8); and, after three days of either uninterrupted sitting or light-intensity walking interruptions (n = 5). Contraction- and insulin-mediated glucose uptake signaling pathways as well as changes in oxidative phosphorylation proteins were examined. We showed that 1) both interventions reduce postprandial glucose concentration, 2) acute interruptions to sitting over one day stimulate the contraction-mediated glucose uptake pathway, 3) both acute interruptions to sitting with moderate-intensity activity over one day and light-intensity activity over three days induce a transition to modulation of the insulin-signaling pathway, in association with increased capacity for glucose transport. Only the moderate-intensity interruptions resulted in greater capacity for glycogen synthesis and likely for ATP production. These observations contribute to a mechanistic explanation of improved postprandial glucose metabolism with regular interruptions to sitting time, a promising preventive strategy for metabolic diseases. |
| Databáze: | OpenAIRE |
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