Evaluation of incubation time for dermatophytes cultures
| Autor: | Antonio Rezusta, Ana Isabel López-Calleja, Maria Angeles Ruiz, Sonia de la Fuente, Yolanda Gilaberte, María José Revillo, Leticia Alcalá, Matxalen Vidal-García |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Fungal growth Time Factors Epidermophyton floccosum 030106 microbiology Mycology Dermatology Trichophyton rubrum medicine.disease_cause Incubation period Microbiology 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Tinea Trichophyton medicine Incubation biology Arthrodermataceae Epidermophyton General Medicine biology.organism_classification Infectious Diseases Dermatophyte Visual observation |
| Zdroj: | Mycoses. 59:416-418 |
| ISSN: | 0933-7407 |
| DOI: | 10.1111/myc.12484 |
| Popis: | In general, it is recommended to incubate dermatophytes cultures for a minimum of 4 weeks. Several aspects of routine fungal cultures should be evaluated in order to implement appropriate and necessary changes. The aim of this study was to determine the optimum incubation time for routine dermatophytes cultures, analysing the time to find first fungal growth by visual observation. We recorded the time when the initial growth was detected for all dermatophyte isolates during a 4-year period. A total of 5459 dermatophyte cultures were submitted to our laboratory. From the total cultures, only 16 (1.42%) isolates were recovered over/after 17 days of incubation and only three dermatophyte species were recovered over 17 days. Fourteen isolates belong to Trichophyton rubrum, one isolate to Trichophyton mentagrophytes complex and one isolate to Epidermophyton floccosum. We concluded that an incubation period of 17 days is enough to establish a microbiological diagnosis of dermatophytosis. |
| Databáze: | OpenAIRE |
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