Relationship of cellular transmigration and airway response after allergen challenge
| Autor: | Christopher Maxwell, Michael Waldman, Alan R. Leff, I. M. Ndukwu, Edward T. Naureckas |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Pulmonary and Respiratory Medicine
Hypersensitivity Immediate Male Allergy Neutrophils Bronchi Cell Count Critical Care and Intensive Care Medicine medicine.disease_cause Bronchial Provocation Tests Leukocyte Count Allergen Eosinophil migration Cell Movement Forced Expiratory Volume Bronchoscopy medicine Eosinophilia Humans Methacholine Chloride Asthma Skin Tests medicine.diagnostic_test business.industry Respiratory disease respiratory system Eosinophil Allergens medicine.disease Eosinophils Pulmonary Alveoli Bronchoalveolar lavage medicine.anatomical_structure Immunology Female medicine.symptom business Bronchoalveolar Lavage Fluid |
| Zdroj: | American journal of respiratory and critical care medicine. 160(5 Pt 1) |
| ISSN: | 1073-449X |
| Popis: | We examined the relationship between eosinophil migration into the bronchoalveolar space and change in FEV(1) after endobronchial allergen challenge (EBAC) in atopic asthmatic (AA) and atopic nonasthmatic (ANA) subjects. The purpose of this study was to obtain continuous, intrasubject controlled assessment of the relationship between cell migration in control and allergen-challenged segments in the same individuals over 96 h. In AA subjects, the eosinophil (Eos) count in the bronchoalveolar lavage fluid (BALF) increased from a baseline of 7,896 +/- 3,865 to 416,476 +/- 231,012 Eos/ml by 72 h (p = 0.001) in the challenged segment post-EBAC. For ANA subjects, the postsegmental challenge count was 29,874 +/- 474 Eos/ml (p = 0.03 versus baseline and p0.05 AA peak versus ANA peak). In both groups, there was a comparable decrease in peripheral blood eosinophil count beginning 5 h after challenge, which resolved at 24 h. In AA subjects, 416,476 +/- 231,012 Eos/ml was obtained from the allergen-challenged segment and 23,522 +/- 8,298 Eos/ml was obtained from the sham-challenged segment (p0.001) at 72 h. In contrast, there was no difference in the Eos count obtained from the BALF between the antigen- and sham-challenged segments of ANA subjects. We also found that increased airway neutrophils were present in equal numbers in allergen-challenged and sham-challenged segments in both AA and ANA subjects. We conclude that augmented eosinophil migration after EBAC is a characteristic of atopic asthma and is not present in atopic subjects who do not have asthma. We find that BAL eosinophilia in ANA patients as well as neutrophilia in both ANA and AA subjects are nonspecific consequences of bronchoscopy. Finally, we find no relationship between specific airway eosinophil migration into the BALF and FEV(1)72 h after challenge; however, at 96 h, there is a substantial decrease in FEV(1) that accompanies BALF eosinophilia. |
| Databáze: | OpenAIRE |
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