The Arabidopsis PILZ group genes encode tubulin-folding cofactors orthologues required for cell division but not cell growth
| Autor: | Gerd Jürgens, York-Dieter Stierhof, Ulrike Mayer, Heinz Schwarz, Frank Küttner, Bernd Geiges, Tobias Pacher, Loïc Lepiniec, Susanne Trautmann, Bianca Priester, Christoph Maulbetsch, Katharina Steinborn |
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| Přispěvatelé: | Biologie des Semences (LBS), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G) |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
0106 biological sciences
Protein Folding Saccharomyces cerevisiae Proteins Cell division Microtubule-associated protein [SDV]Life Sciences [q-bio] Molecular Sequence Data Arabidopsis Biology Genes Plant 01 natural sciences 03 medical and health sciences Tubulin Microtubule Genetics Humans Cloning Molecular Cytoskeleton Actin 030304 developmental biology 0303 health sciences Sequence Homology Amino Acid Arabidopsis Proteins Qa-SNARE Proteins Cell growth Membrane Proteins Membrane Transport Proteins Cell biology Protein Transport Mutation Seeds biology.protein Genes Lethal Microtubule-Associated Proteins Sequence Alignment Cell Division Cytokinesis Research Paper 010606 plant biology & botany Developmental Biology |
| Zdroj: | Genes and Development Genes and Development, Cold Spring Harbor Laboratory Press, 2002, 16, pp.959-971. ⟨10.1101/gad.221702⟩ |
| ISSN: | 0890-9369 |
| DOI: | 10.1101/gad.221702⟩ |
| Popis: | Plant microtubules are organized into specific cell cycle-dependent arrays that have been implicated in diverse cellular processes, including cell division and organized cell expansion. Mutations in fourArabidopsis genes collectively called the PILZ group result in lethal embryos that consist of one or a few grossly enlarged cells. The mutant embryos lack microtubules but not actin filaments. Whereas the cytokinesis-specific syntaxin KNOLLE is not localized properly, trafficking of the putative auxin efflux carrier PIN1 to the plasma membrane is normal. The four PILZ group genes were isolated by map-based cloning and are shown to encode orthologs of mammalian tubulin-folding cofactors (TFCs) C, D, and E, and associated small G-protein Arl2 that mediate the formation of α/β-tubulin heterodimers in vitro. The TFC C ortholog, PORCINO, was detected in cytosolic protein complexes and did not colocalize with microtubules. Another gene with a related, although weaker, embryo-lethal phenotype,KIESEL, was shown to encode a TFC A ortholog. Our genetic ablation of microtubules shows their requirement in cell division and vesicle trafficking during cytokinesis, whereas cell growth is mediated by microtubule-independent vesicle trafficking to the plasma membrane during interphase. |
| Databáze: | OpenAIRE |
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