A convergent synthetic platform for dual anticancer drugs functionalized by reduced graphene nanocomposite delivery for hepatocellular cancer
| Autor: | Jian Wei, Zeran Yang, Yanjing Han, Long Jin, Zhiyuan Zhang, Tianhao Su, Haining Fan |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Carcinoma Hepatocellular Cell Survival Chemistry Pharmaceutical Pharmaceutical Science Antineoplastic Agents RM1-950 Nanocomposites in vivo animal model Mice medicine Animals Humans In vivo animal model Polyhydroxyethyl Methacrylate Drug Carriers Mice Inbred BALB C Hepatocellular cancer business.industry Liver Neoplasms graphene apoptosis Cancer Hep G2 Cells hepatocellular carcinoma General Medicine medicine.disease Metformin digestive system diseases Drug Liberation Chemotherapy Drugs Hepatocellular carcinoma Cancer research Graphite Magnetic Iron Oxide Nanoparticles Therapeutics. Pharmacology Caco-2 Cells Cisplatin Reactive Oxygen Species business combination drugs Research Article |
| Zdroj: | Drug Delivery, Vol 28, Iss 1, Pp 1982-1994 (2021) Drug Delivery article-version (VoR) Version of Record |
| ISSN: | 1521-0464 1071-7544 |
| DOI: | 10.1080/10717544.2021.1974606 |
| Popis: | Hepatocellular carcinoma (HCC) is widespread cancer with a high degree of morbidity and mortality in individuals worldwide and a serious concern for its resistance to present chemotherapy drugs. In this investigation, the combination of cisplatin (CPT) and metformin (MET) to kill the HepG2 and caco-2 cells was developed into a new pH-responding magnetic nanocomposite based on reduced graphene oxide. Polyhydroxyethyl methacrylic (PHEA) was then linked employing grafting from approach to the reduced graphene oxide by ATRP polymerization (Fe3O4@rGO-G-PSEA). FT-IR, SEM, XRD, DLS, and TGA analyses evaluated physicochemical characteristics of the nanocomposite. In addition, the cellular uptake property of the nanocomposites was examined by the HepG2 cells. The outcomes of cell viability results indicate that the nanoparticles loaded with MET&CPT showed the lowest concentration rate of HepG2 and Caco-2 cells compared to the drug-loaded single nanocomposite groups and free drugs. The histological analysis has demonstrated relatively safe and does not produce different stress such as swelling and inflammation of the mice organs. Our results show the enhancement in cytotoxicity in HepG2 and Cocoa-2 cells by MET and CPT graphene oxide-based nanocomposite by promoting apoptotic response. Moreover, Fe3O4@rGO-G-PSEA showed potent in vivo antitumor efficacy but showed no adverse toxicity to normal tissues. Together, this study can provide insight into how surface embellishment may tune these nanocomposites' tumor specificity and provide the basis for developing anticancer efficacy. |
| Databáze: | OpenAIRE |
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