A quantitative analysis of electrolyte exchange in the salivary duct
| Autor: | James E. Melvin, Kate Patterson, Edmund J. Crampin, James Sneyd, David I. Yule, Marcelo A. Catalán |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Saliva
Physiology Submandibular duct Cystic Fibrosis Transmembrane Conductance Regulator Acinar Cells Models Biological Sodium Channels Membrane Potentials Electrolytes Mice stomatognathic system Chlorides Mucosal Biology Physiology (medical) medicine Animals Salivary Ducts Ion transporter Membrane potential Ion Transport Hepatology biology Salivary gland Chemistry Sodium Gastroenterology Membrane transport Cystic fibrosis transmembrane conductance regulator Bicarbonates medicine.anatomical_structure Biochemistry biology.protein Biophysics Potassium |
| Popis: | A healthy salivary gland secretes saliva in two stages. First, acinar cells generate primary saliva, a plasma-like, isotonic fluid high in Na+ and Cl−. In the second stage, the ducts exchange Na+ and Cl− for K+ and HCO3−, producing a hypotonic final saliva with no apparent loss in volume. We have developed a tool that aims to understand how the ducts achieve this electrolyte exchange while maintaining the same volume. This tool is part of a larger multiscale model of the salivary gland and can be used at the duct or gland level to investigate the effects of genetic and chemical alterations. In this study, we construct a radially symmetric mathematical model of the mouse salivary gland duct, representing the lumen, the cell, and the interstitium. For a given flow and primary saliva composition, we predict the potential differences and the luminal and cytosolic concentrations along a duct. Our model accounts well for experimental data obtained in wild-type animals as well as knockouts and chemical inhibitors. Additionally, the luminal membrane potential of the duct cells is predicted to be very depolarized compared with acinar cells. We investigate the effects of an electrogenic vs. electroneutral anion exchanger in the luminal membrane on concentration and the potential difference across the luminal membrane as well as how impairing the cystic fibrosis transmembrane conductance regulator channel affects other ion transporting mechanisms. Our model suggests the electrogenicity of the anion exchanger has little effect in the submandibular duct. |
| Databáze: | OpenAIRE |
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