Kdm6a overexpression improves the development of cloned mouse embryos
| Autor: | Yu-Wei Zhang, Ruizhen Sun, Xiang Huang, Guang-Yu Bai, Lei Lei, Si-Hang Song, Xing-Wei Huang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Nuclear Transfer Techniques Somatic cell Cloning Organism Mice Inbred Strains Biology DNA Ribosomal Histones 03 medical and health sciences X Chromosome Inactivation Transcription (biology) medicine Animals Humans Blastocyst Histone Demethylases Messenger RNA Cumulus Cells Lysine Gene Expression Regulation Developmental Embryo Cell Biology Blastomere Cell biology HEK293 Cells 030104 developmental biology medicine.anatomical_structure embryonic structures Somatic cell nuclear transfer Female Reprogramming Developmental Biology |
| Zdroj: | Zygote. 26:24-32 |
| ISSN: | 1469-8730 0967-1994 |
| Popis: | SummarySomatic cell nuclear transfer (SCNT) is an important technique for life science research. However, most SCNT embryos fail to develop to term due to undefined reprogramming defects. Here, we show that abnormal Xi occurs in somatic cell NT blastocysts, whereas in female blastocysts derived from cumulus cell nuclear transfer, both X chromosomes were inactive. H3K27me3 removal by Kdm6a mRNA overexpression could significantly improve preimplantation development of NT embryos, and even reached a 70.2% blastocyst rate of cleaved embryos compared with the 38.5% rate of the control. H3K27me3 levels were significantly reduced in blastomeres from cloned blastocysts after overexpression of Kdm6a. qPCR indicated that rDNA transcription increased in both NT embryos and 293T cells after overexpression of Kdm6a. Our findings demonstrate that overexpression of Kdm6a improved the development of cloned mouse embryos by reducing H3K27me3 and increasing rDNA transcription. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|