Current LC-MS-based strategies for characterization and quantification of antibody-drug conjugates
| Autor: | Jun Qu, Shihan Huo, Chao Xue, Bo An, Xiaoyu Zhu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Review paper
Pharmaceutical Science Nanotechnology 02 engineering and technology Pharmacy Molecular resolution 01 natural sciences Analytical Chemistry Drug-to-antibody ratio (DAR) Liquid chromatography–mass spectrometry High complexity Drug Discovery Electrochemistry Spectroscopy Liquid chromatography-mass spectrometry (LCMS) Chemistry 010401 analytical chemistry lcsh:RM1-950 Intact protein Antibody-drug conjugate (ADC) 021001 nanoscience & nanotechnology Method development 0104 chemical sciences body regions lcsh:Therapeutics. Pharmacology 0210 nano-technology |
| Zdroj: | Journal of Pharmaceutical Analysis Journal of Pharmaceutical Analysis, Vol 10, Iss 3, Pp 209-220 (2020) |
| ISSN: | 2214-0883 2095-1779 |
| Popis: | The past few years have witnessed enormous progresses in the development of antibody-drug conjugates (ADCs). Consequently, comprehensive analysis of ADCs in biological systems is critical in supporting discovery, development and evaluation of these agents. Liquid chromatography-mass spectrometry (LC-MS) has emerged as a promising and versatile tool for ADC analysis across a wide range of scenarios, owing to its multiplexing ability, rapid method development, as well as the capability of analyzing a variety of targets ranging from small-molecule payloads to the intact protein with a high, molecular resolution. However, despite this tremendous potential, challenges persist due to the high complexity in both the ADC molecules and the related biological systems. This review summarizes the up-to-date LC-MS-based strategies in ADC analysis and discusses the challenges and opportunities in this rapidly-evolving field. Graphical abstract Image 1 Highlights • ADC is an important class of anti-cancer agents but optimal analytical approaches remain elusive despite years of efforts. • LC-MS shows promising capability for protein analysis at peptide, subunit and intact levels, as well as payload analysis. • LC-MS is a powerful tool for ADC DAR measurement because of its molecular-level resolution and the matrix-independency. • A score of novel LC-MS-based techniques have emerged in ADC analysis, both quantitatively and qualitatively. |
| Databáze: | OpenAIRE |
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