Additional file 6 of Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of different display systems

Autor: Nicchi, Sonia, Giuliani, Maria, Giusti, Fabiola, Pancotto, Laura, Maione, Domenico, Delany, Isabel, Galeotti, Cesira L., Brettoni, Cecilia
Rok vydání: 2021
DOI: 10.6084/m9.figshare.13698224
Popis: Additional file 6: Figure S3. FACS analysis of NmBamE fused to the InaK delivery system in E. coli, at 25°C using the polyclonal anti-NmBamE antibodies. E. coli BL21DE3 and E. coli T7ExpressIq (pET15b) expressing InaK fused the N. meningitidis lipoprotein BamE at 25°C (A and B, respectively) were incubated with the polyclonal anti-NmBamE antibodies. The grey areas represent the fluorescence signals obtained with the control (BL21DE3-pET15b ∅ or T7ExpressIq pET15b ∅, panels A and B, respectively). The Light Blue coloured line represents the fused forms of the NmBamE lipoprotein.
Databáze: OpenAIRE