Requirement for frzb and fzd7a in cranial neural crest convergence and extension mechanisms during zebrafish palate and jaw morphogenesis
| Autor: | Tatiana Hoyos, Michael Grimaldi, Eric C. Liao, Valeriy Shubinets, William Ka Fai Tse, George N. Kamel, Max L. Dougherty, Lucie Rochard, Yawei Kong |
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| Rok vydání: | 2013 |
| Předmět: |
Frizzled
Embryo Nonmammalian Morphogenesis Apoptosis Receptors Cell Surface Chondrocytes Cranial neural crest Animals Humans Craniofacial Molecular Biology Zebrafish Body Patterning Cell Proliferation Glycoproteins Homeodomain Proteins biology Palate Intracellular Signaling Peptides and Proteins Wnt signaling pathway Gene Expression Regulation Developmental Neural crest Epistasis Genetic Cell Biology Anatomy Zebrafish Proteins biology.organism_classification Cell biology Wnt Proteins HEK293 Cells Jaw Frzb Neural Crest Transcription Factors Developmental Biology |
| Zdroj: | Developmental Biology. 381:423-433 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2013.06.012 |
| Popis: | Regulation of convergence and extension by wnt-frizzled signaling is a common theme in embryogenesis. This study examines the functional requirements of frzb and fzd7a in convergence and extension mechanisms during craniofacial development. Using a morpholino knockdown approach, we found that frzb and fzd7a are dispensable for directed migration of the bilateral trabeculae, but necessary for the convergence and extension of the palatal elements, where the extension process is mediated by chondrocyte proliferation, morphologic change and intercalation. In contrast, frzb and fzd7a are required for convergence of the mandibular prominences, where knockdown of either frzb or fzd7a resulted in complete loss of lower jaw structures. Further, we found that bapx1 was specifically downregulated in the wnt9a/frzb/fzd7a morphants, while general neural crest markers were unaffected. In addition, expression of wnt9a and frzb was also absent in the edn-/- mutant. Notably, over-expression of bapx1 was sufficient to partially rescue mandibular elements in the wnt9a/frzb/fzd7a morphants, demonstrating genetic epistasis of bapx1 acting downstream of edn1 and wnt9a/frzb/fzd7a in lower jaw development. This study underscores the important role of wnt-frizzled signaling in convergence and extension in palate and craniofacial morphogenesis, distinct regulation of upper vs. lower jaw structures, and integration of wnt-frizzled with endothelin signaling to coordinate shaping of the facial form. |
| Databáze: | OpenAIRE |
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