Modulation of pro-apoptotic effects and mitochondrial potential on B16F10 cells by DODAC/PHO-S liposomes
| Autor: | Gilberto Orivaldo Chierice, Durvanei Augusto Maria, Salvador Claro Neto, José Roberto de Assis Santos Filho, Arthur Cássio de Lima Luna, Henrique Hesse |
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| Rok vydání: | 2018 |
| Předmět: |
inorganic chemicals
0301 basic medicine Receptor expression Melanoma Experimental lcsh:Medicine Apoptosis Mitochondrion General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Adjuvants Immunologic Cell Line Tumor Animals Nanotechnology Cytotoxic T cell lcsh:Science (General) Cytotoxicity Melanoma Phosphoethanolamine lcsh:QH301-705.5 Caspase Liposome biology Cytotoxins urogenital system Chemistry Cytochrome c lcsh:R General Medicine Mitochondria Cell biology Quaternary Ammonium Compounds Research Note 030104 developmental biology lcsh:Biology (General) Ethanolamines 030220 oncology & carcinogenesis Liposomes biology.protein lcsh:Q1-390 |
| Zdroj: | BMC Research Notes, Vol 11, Iss 1, Pp 1-6 (2018) BMC Research Notes |
| ISSN: | 1756-0500 |
| Popis: | Objective We aimed to evaluate the potential of DODAC/PHO-S liposomes on the modulation of the expression of pro-apoptotic proteins, loss of lysosomal integrity and the mitochondrial electrical potential, compared with phosphoethanolamine. Results The results of this study demonstrate that DODAC/PHO-S liposomes have exhibited broad cytotoxic potential in B16F10 murine melanoma cells, with significantly greater proportions than treatment with PHO-S. The treatment with the DODAC/PHO-S 2.0 mM liposomal formulation was more efficient in decreasing mitochondrial electrical potential at the same concentrations and treatment time than PHO-S The liposomal formulation DODAC/PHO-S (2.0 mM) was more efficient to promote morphological changes in the cells, without presenting intact lysosomes, at the same time of treatment and concentration as PHO-S Our results demonstrated that the liposomal formulation increased DR4 receptor expression and activated caspases 8 and 3, resulting in the release of cytochrome c in B16F10 tumour cells, when compared to treatment with PHO-S The data obtained prove that the use of DODAC as carrier can maximize the cytotoxic effects of PHO-S This was demonstrated by the translocation of cytochrome c to the cytoplasm and activation of caspase-3 and 8, decreasing the mitochondrial electrical potential and generating morphological changes, in B16F10 cells. |
| Databáze: | OpenAIRE |
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