Learning-induced plasticity in the barrel cortex is disrupted by inhibition of layer 4 somatostatin-containing interneurons
| Autor: | Dominik Kanigowski, Malgorzata Kossut, R. Zakrzewska, Monika Liguz-Lecznar, A. Posluszny, G. Dobrzanski, A. Lukomska, Joanna Urban-Ciecko |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Vasoactive intestinal peptide
Stimulation Plasticity Mice Interneurons Membrane Transport Modulators medicine Animals Learning GABAergic Neurons Molecular Biology Neuronal Plasticity biology Chemistry Classical conditioning Neural Inhibition Cell Biology Somatosensory Cortex Barrel cortex medicine.anatomical_structure Somatostatin Disinhibition Cerebral cortex Vibrissae biology.protein Conditioning medicine.symptom Neuroscience Parvalbumin |
| DOI: | 10.1101/2020.05.11.087791 |
| Popis: | Gaba-ergic neurons are a diverse cell class with extensive influence over cortical processing, but their role in experience-dependent plasticity is not completely understood. Here we addressed the role of cortical somatostatin- (SOM-INs) and vasoactive intestinal polypeptide- (VIP-INs) containing interneurons in a Pavlovian conditioning where stimulation of the vibrissae is used as a conditioned stimulus and tail shock as unconditioned one. This procedure induces a plastic change observed as an enlargement of the cortical functional representation of vibrissae activated during conditioning. Using layer-targeted, cell-selective DREADD transductions, we examined the involvement of SOM-INs and VIP-INs activity in learning-related plastic changes. Under optical recordings, we injected DREADD-expressing vectors into layer IV (L4) barrels or layer II/III (L2/3) areas corresponding to the activated vibrissae. The activity of the interneurons was modulated during all conditioning sessions, and functional 2-deoxyglucose (2DG) maps were obtained 24 h after the last session. In mice with L4 but not L2/3 SOM-INs suppressed during conditioning, the plastic change of whisker representation was absent. The behavioral effect of conditioning was disturbed. Both L4 SOM-INs excitation and L2/3 VIP-INs inhibition during conditioning did not affect the plasticity or the conditioned response. We found the activity of L4 SOM-INs is indispensable in the formation of learning-induced plastic change. We propose that L4 SOM-INs may provide disinhibition by blocking L4 parvalbumin interneurons, allowing a flow of information into upper cortical layers during learning. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|