Progenitor cells in the blood and marrow of patients with chronic phase chronic myeloid leukaemia respond differently to macrophage inflammatory protein-1 alpha
| Autor: | M.Y. Gordon, N. Nirsimloo |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
Myeloid medicine.medical_treatment CFU-GM Bone Marrow Cells Biology In Vitro Techniques Monocytes Colony-Forming Units Assay hemic and lymphatic diseases Leukemia Myelogenous Chronic BCR-ABL Positive medicine Macrophage Humans Progenitor cell Chemokine CCL4 Macrophage inflammatory protein Cells Cultured Monokines Hematology Macrophage Inflammatory Proteins Hematopoiesis medicine.anatomical_structure Cytokine Oncology Immunology Cancer research Cytokines Bone marrow Stem cell Granulocytes |
| Zdroj: | Leukemia research. 19(5) |
| ISSN: | 0145-2126 |
| Popis: | Macrophage inflammatory protein-1 alpha (MIP-1 alpha) is a negative regulator of normal haemopoietic stem cell proliferation. Insensitivity to MIP-1 alpha of progenitor cells in chronic myeloid leukaemia (CML) could, therefore, explain myeloid expansion in this disease. We compared the effects of MIP-1 alpha on progenitor cells in normal marrow and in the blood and marrow of patients with chronic phase CML. Plastic-adherent precursors of granulocyte-macrophage colony-forming cells (P delta progenitors) are very primitive progenitor cells and are detected by incubating them for 1 week in liquid culture and assaying the CFU-GM released into the supernatant. Direct CFU-GM assays were also used in this study. Daily addition of 300 ng/ml/day of MIP-1 alpha to P delta progenitor assays of normal marrow cells suppressed CFU-GM production by 50% and in CML bone marrow P delta cultures by 20-30%. The response of CML blood P delta progenitors was heterogeneous. In five of nine cases, CFU-GM production was doubled in the presence of MIP-1 alpha and in four of nine cases, it was reduced. Addition of 100-500 ng MIP-1 alpha to direct assays of CFU-GM stimulated colony formation by normal marrow and CML blood cells to a similar extent. |
| Databáze: | OpenAIRE |
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