Convergent differentiation: myeloid and lymphoid pathways to murine plasmacytoid dendritic cells
| Autor: | Lynn M. Corcoran, Ken Shortman, Li Wu, Priyanka Sathe, David Vremec |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adoptive cell transfer
Myeloid Immunology Gene Rearrangement B-Lymphocyte Heavy Chain Oligonucleotides Alpha interferon Bone Marrow Cells Cell Separation Biology Biochemistry Recombination-activating gene Immunophenotyping Mice hemic and lymphatic diseases medicine Animals Cell Lineage Lymphopoiesis Cells Cultured Homeodomain Proteins Myelopoiesis Regulation of gene expression B-Lymphocytes Gene Expression Regulation Developmental Interferon-alpha Membrane Proteins hemic and immune systems Dendritic Cells Cell Biology Hematology Gene rearrangement Adoptive Transfer Clone Cells Specific Pathogen-Free Organisms Cell biology Mice Inbred C57BL medicine.anatomical_structure Radiation Chimera CpG Islands |
| Zdroj: | Blood. 121:11-19 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2012-02-413336 |
| Popis: | The developmental origin of IFN-producing plasmacytoid dendritic cells (pDCs) has been uncertain. In the present study, we tracked the development of pDCs in cultures of BM precursors stimulated with Flt3 ligand. Common myeloid precursors (CMPs) produced both conventional DCs (cDCs) and pDCs via the DC-restricted common DC precursor. Common lymphoid precursors (CLPs) produced only a few cDCs with variable efficiency, but produced pDCs via a transient intermediate precursor with B-cell potential. The pDCs of both origins produced IFN-α when stimulated with CpG oligonucleotides. The pDCs of CLP origin showed evidence of past RAG1 expression and had D-J rearrangements in IgH genes. Most pDCs and all cDCs of CMP origin lacked these signs of a lymphoid past. However, in these cultures, some pDCs of CMP origin showed evidence of past RAG1 expression and had D-J IgH gene rearrangements; most of these derived from a subset of CMPs already expressing RAG1. |
| Databáze: | OpenAIRE |
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