The effect of omalizumab on nasal allergic inflammation
| Autor: | Ellinor Ädelroth, Thomas Sandström, Sabina Rak, Kenneth Holmberg, Hans Gustafsson, Monica Arvidsson, Halina Plewako, Iolanda Oancea, Karin Petruson |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male Allergy Biopsy Immunology Omalizumab Granulocyte Immunoglobulin E Antibodies Monoclonal Humanized Allergic inflammation Leukocyte Count Anti-Allergic Agents medicine Immunology and Allergy Humans Inflammation medicine.diagnostic_test biology business.industry Antibodies Monoclonal Rhinitis Allergic Seasonal Eosinophil Middle Aged medicine.disease Immunohistochemistry Antibodies Anti-Idiotypic Eosinophils Nasal Mucosa medicine.anatomical_structure Treatment Outcome biology.protein Female Antibody business medicine.drug |
| Zdroj: | The Journal of allergy and clinical immunology. 110(1) |
| ISSN: | 0091-6749 |
| Popis: | Background: In sensitized patients, coupling between IgE and FcϵRI receptors on mast cells leads to release of proinflammatory mediators and a subsequent influx of inflammatory cells to the affected organ. Omalizumab (Xolair; formerly rhuMAb-E25) binds to circulating IgE, thus preventing induction of the allergic process. Objective: We investigated the effect of treatment with omalizumab on seasonal allergic rhinitis and related changes in inflammatory cell numbers in nasal biopsy specimens. Methods: Patients were randomized to treatment with omalizumab or placebo before the pollen season; the treatment was started and continued during season. Symptoms and use of medication were recorded, and blood samples and nasal biopsy specimens were obtained before and during season. Immunocytochemistry was performed on biopsy sections through use of the following antibodies: anti-CD4, CD8 (T lymphocytes), EG2, and anti-eosinophil peroxidase (eosinophils), anti-tryptase (mast cells), human neutrophil lipocalin (neutrophils), and antibodies against IgE and FcϵRI. Results: During the season, blood eosinophils increased in placebo-treated patients but not in omalizumab-treated patients ( P = .01); the difference between the treatment groups was significant ( P = .04). Free IgE in serum decreased significantly ( P = .0002) in omalizumab-treated patients but not in placebo-treated patients; the difference between the groups was significant ( P = .0001). In nasal biopsy specimens, the number of eosinophil peroxidase-positive staining cells increased in the placebo-treated patients ( P = .003) but not in the actively treated patients during the season; the difference between the groups was significant ( P = .0001). The number of IgE + staining cells decreased significantly in the omalizumab group during the season in comparison with the placebo group ( P = .04). Conclusion: The clinical benefit of treatment with omalizumab is associated with an anti-inflammatory effect on cellular markers in blood and nasal tissue. (J Allergy Clin Immunol 2002;110:68-71.) |
| Databáze: | OpenAIRE |
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