Local Preirradiation of Infarcted Cardiac Tissue Substantially Enhances Cell Engraftment
| Autor: | Javier Linares, Leyre López-Muneta, Purificación Ripalda-Cemboráin, Xabier L. Aranguren, Luis Isaac Ramos, Miriam Bobadilla, Arantxa Baraibar-Churio, Felipe Prosper, Gloria Abizanda, Ana Pérez-Ruiz, E. Iglesias, Xonia Carvajal-Vergara, Giulia Coppiello |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
QH301-705.5 Cell Brachytherapy Induced Pluripotent Stem Cells Endogeny Catalysis Inorganic Chemistry Cell therapy Rats Sprague-Dawley cell engraftment Mice medicine Animals Myocardial infarction Physical and Theoretical Chemistry Biology (General) Induced pluripotent stem cell Ischemic Preconditioning Molecular Biology QD1-999 Spectroscopy Cells Cultured Cardiotoxicity business.industry Communication Organic Chemistry Skeletal muscle Heart General Medicine medicine.disease Computer Science Applications Rats Chemistry medicine.anatomical_structure myocardial infarction Cancer research local irradiation Bone marrow cell therapy business Stem Cell Transplantation |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 9126, p 9126 (2021) International Journal of Molecular Sciences |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | The success of cell therapy for the treatment of myocardial infarction depends on finding novel approaches that can substantially implement the engraftment of the transplanted cells. In order to enhance cell engraftment, most studies have focused on the pretreatment of transplantable cells. Here we have considered an alternative approach that involves the preconditioning of infarcted heart tissue to reduce endogenous cell activity and thus provide an advantage to our exogenous cells. This treatment is routinely used in other tissues such as bone marrow and skeletal muscle to improve cell engraftment, but it has never been taken in cardiac tissue. To avoid long-term cardiotoxicity induced by full heart irradiation we developed a rat model of a catheter-based heart irradiation system to locally impact a delimited region of the infarcted cardiac tissue. As proof of concept, we transferred ZsGreen+ iPSCs in the infarcted heart, due to their ease of use and detection. We found a very significant increase in cell engraftment in preirradiated rats. In this study, we demonstrate for the first time that preconditioning the infarcted cardiac tissue with local irradiation can substantially enhance cell engraftment. |
| Databáze: | OpenAIRE |
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