The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes
| Autor: | Thomas F. Meyer, Anne Stern, K. S. Bhat, S. G. Morrison, Eva-Maria Kupsch, John Swanson, O. Barrera, Carol P. Gibbs, Fritz Jähnig |
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| Rok vydání: | 1991 |
| Předmět: |
Molecular Sequence Data
Gene Expression Molecular cloning Biology Microbiology Gene expression Gene duplication Escherichia coli Gene family Amino Acid Sequence Cloning Molecular Molecular Biology Gene Southern blot Genetics Recombination Genetic Antigens Bacterial Base Sequence Nucleic acid sequence Genetic Variation Antigenic Variation Biological Evolution Neisseria gonorrhoeae genomic DNA Blotting Southern Genes Bacterial Multigene Family Bacterial Outer Membrane Proteins |
| Zdroj: | Molecular microbiology. 5(8) |
| ISSN: | 0950-382X |
| Popis: | Summary Variants of Neisseria gonorrhoeae MS11 show distinct colony morphologies because of the expression of a class of surface components called opacity (Opa, PII) proteins. Southern analyses combined with molecular cloning of genomic DNA from a single variant of MS11 has identified 11 opa genes contained in separate loci. These opa genes code for distinct opacity proteins which are distinguishable at their variable domains. The opa gene analyses were also extended to divergent variants of MS11. These studies have shown that, during in vitro and in vivo culture, 10 of the 11 opa genes did not undergo significant change in their primary sequence. However, in these variants, one gene (opaE) underwent non-reciprocat inter-opa recombinations to generate newer Opa variants. Phylogenic analysis of the opa gene sequences suggests that the opa gene family have evolved by a combination of gene duplication, gene replacement and partial inter-opa recombination events. |
| Databáze: | OpenAIRE |
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