| Autor: |
Wangyu Zhu, Yun Yu, Kexin Fang, Sisi Xiao, Lianli Ni, Changtian Yin, Xiangjie Huang, Xinchen Wang, Yongkui Zhang, Han‐Bo Le, Ri Cui |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Cancer Medicine. 12:4590-4604 |
| ISSN: |
2045-7634 |
| DOI: |
10.1002/cam4.5309 |
| Popis: |
RNA-binding protein Quaking-5 (QKI-5), a major isoform of QKIs, inhibits tumor progression in non-small cell lung cancer (NSCLC). However, the underlying molecular mechanisms of QKI-5 in the cell cycle of NSCLC are still largely unknown.MTT, flow cytometry, and colony formation assays were used to investigate cellular phenotypic changes. Mice xenograft model was used to evaluate the antitumor activities of QKI-5. Co-immunoprecipitation, RNA immunoprecipitation (RIP), and RIP sequencing were used to investigate protein-protein interaction and protein-mRNA interaction.The QKI-5 expression was downregulated in NSCLC tissues compared with that in paired normal adjacent lung tissues. Overexpression of QKI-5 inhibited NSCLC cell proliferative and colony forming ability. In addition, QKI-5 induced cell cycle arrest at G0/G1 phase through upregulating p21Our results suggest that the miR-31/QKI-5/p21-CDK4-CDK6 axis might have critical functions in the progression of NSCLC, and targeting this axis could serve as a potential therapeutic strategy for NSCLC. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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