DHICA oxidase activity of TRP1 and interactions with other melanogenic enzymes
| Autor: | Katsuhiko Tsukamoto, Timothy Brewington, Alison J. Winder, Brian Potterf, Vincent J. Hearing, Kazunori Urabe, Genji Imokawa, Takeshi Kobayashi |
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| Rok vydání: | 1994 |
| Předmět: |
Indoles
Tyrosinase Clinical Biochemistry Melanoma Experimental Plant Science Biology Transfection Catalysis Melanin chemistry.chemical_compound Mice Enzyme Stability Animals Indolequinones Gene chemistry.chemical_classification Melanins Membrane Glycoproteins Monophenol Monooxygenase Pigmentation Structural gene DHICA Quinones Proteins Cell Biology Fibroblasts Enzyme chemistry Biochemistry Dopachrome Oxidoreductases Agronomy and Crop Science Developmental Biology |
| Zdroj: | Pigment cell research. 7(4) |
| ISSN: | 0893-5785 |
| Popis: | Tyrosinase-related protein 1 (TRP1) maps to the brown locus in mice. Although the specific function of TRP1 has been in dispute, mutations in its structural gene result in the formation of brown rather than black melanin. We have investigated the melanogenic function of TRP1 by using immune-affinity purification of the protein and also by using transfection of its gene into fibroblasts to study its characteristics. We show that TRP1 has the ability to oxidize DHICA, a melanogenic intermediate derived from DOPAchrome. In addition, TRP1 has the ability to interact with tyrosinase and significantly stabilize the latter's catalytic function. |
| Databáze: | OpenAIRE |
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