Effect of preparation method and cholesterol on drug encapsulation studies by phospholipid liposomes
| Autor: | Seyda Bucak, Fatma Melis Cagdas, Nurettin Ertugral, Naz Zeynep Atay |
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| Rok vydání: | 2010 |
| Předmět: |
Liposome
Chromatography Chemistry Cholesterol Chemistry Pharmaceutical Drug Compounding Bilayer Vesicle Sonication Phospholipid Cytochromes c Ultrafiltration Pharmaceutical Science Phosphatidylglycerols General Medicine chemistry.chemical_compound Liposomes Vitamin E Extrusion Particle size Particle Size Dimyristoylphosphatidylcholine Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Pharmaceutical Development and Technology. 16:408-414 |
| ISSN: | 1097-9867 1083-7450 |
| Popis: | Unilamellar liposomes, prepared from synthetic lipid mixture of DMPC and DMPG either by sonication or extrusion, were used to entrap water soluble and water insoluble molecules to investigate the efficacy of encapsulation by different liposome preparation methods. In the case of entrapment of hydrophilic protein cytochrome-C, the solutions were subjected to a series of ultrafiltration steps to eliminate any free protein outside the vesicles. It was observed that the protein could be encapsulated by the vesicles only if cholesterol was present in the bilayer. The release of cytochrome-C was observed spectrophotometrically upon vesicle-breakdown. The amount of protein encapsulated depended on the method of preparation and was found to be 10 times greater in extruded liposomes compared to those produced by sonication. Hydrophobic Vitamin E, on the other hand, could be encapsulated in the liposome bilayer, independently of the presence of cholesterol and the method of preparation. These fundamental results can be used to develop more efficient drug encapsulations and to have better understanding about their release. |
| Databáze: | OpenAIRE |
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