Adult human CD133/1+ kidney cells isolated from papilla integrate into developing kidney tubules

Autor: Vincent H. Gattone, Heather H. Ward, Robert L. Bacallao, Gavin Pickett, Angela Welford, Elsa Romero, Scott A. Ness, Angela Wandinger-Ness, Tamara Roitbak
Rok vydání: 2011
Předmět:
Renopoietic
medicine.medical_specialty
Pathology
Renal cortex
030232 urology & nephrology
Cell Separation
Biology
Article
Colony-Forming Units Assay
Mice
03 medical and health sciences
Organ Culture Techniques
0302 clinical medicine
Antigens
CD
Internal medicine
medicine
Polycystic kidney disease
Animals
Humans
AC133 Antigen
Progenitor cell
Molecular Biology
Renal stem cell
Glycoproteins
ADPKD
Mesenchymal stem cell
030304 developmental biology
Kidney Medulla
0303 health sciences
Kidney
urogenital system
Cell Differentiation
Xanthosine
Kidney disease
Polycystic Kidney
Autosomal Dominant
Metanephric organ culture
medicine.disease
Coculture Techniques
Major duodenal papilla
Adult Stem Cells
Tamm–Horsfall/uromodulin
Kidney Tubules
medicine.anatomical_structure
Endocrinology
Renal papilla
Regenerative medicine
Molecular Medicine
Peptides
Zdroj: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1812:1344-1357
ISSN: 0925-4439
DOI: 10.1016/j.bbadis.2011.01.010
Popis: Approximately 60,000 patients in the United States are waiting for a kidney transplant due to genetic, immunologic and environmentally caused kidney failure. Adult human renal stem cells could offer opportunities for autologous transplant and repair of damaged organs. Current data suggest that there are multiple progenitor types in the kidney with distinct localizations. In the present study, we characterize cells derived from human kidney papilla and show their capacity for tubulogenesis. In situ, nestin(+) and CD133/1(+) cells were found extensively intercalated between tubular epithelia in the loops of Henle of renal papilla, but not of the cortex. Populations of primary cells from the renal cortex and renal papilla were isolated by enzymatic digestion from human kidneys unsuited for transplant and immuno-enriched for CD133/1(+) cells. Isolated CD133/1(+) papillary cells were positive for nestin, as well as several human embryonic stem cell markers (SSEA4, Nanog, SOX2, and OCT4/POU5F1) and could be triggered to adopt tubular epithelial and neuronal-like phenotypes. Isolated papillary cells exhibited morphologic plasticity upon modulation of culture conditions and inhibition of asymmetric cell division. Labeled papillary cells readily associated with cortical tubular epithelia in co-culture and 3-dimensional collagen gel cultures. Heterologous organ culture demonstrated that CD133/1(+) progenitors from the papilla and cortex became integrated into developing kidney tubules. Tubular epithelia did not participate in tubulogenesis. Human renal papilla harbor cells with the hallmarks of adult kidney stem/progenitor cells that can be amplified and phenotypically modulated in culture while retaining the capacity to form new kidney tubules. This article is part of a Special Issue entitled: Polycystic Kidney Disease.
Databáze: OpenAIRE
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