G[alpha]i2 and G[alpha]q expression change in pancreatic tissues and BN52021 effects in rats with severe acute pancreatitis
| Autor: | Li-rong Liu, Shi-Hai Xia, Chun-Xiu Hu, Yao Di, Zhi-ling Zhao, Jin-miao Fang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Endocrinology Diabetes and Metabolism Alpha (ethology) Phase point Gene Expression Platelet Membrane Glycoproteins Receptors G-Protein-Coupled Lactones Endocrinology Internal Medicine medicine Animals RNA Messenger Rats Wistar Pancreas DNA Primers Hepatology Base Sequence Chemistry medicine.disease Molecular biology Rats Ginkgolides Pancreatitis Acute Disease Acute pancreatitis GTP-Binding Protein alpha Subunits Gq-G11 GTP-Binding Protein alpha Subunit Gi2 |
| Zdroj: | Pancreas. 37(2) |
| ISSN: | 1536-4828 |
| Popis: | OBJECTIVE To investigate change of G[alpha]i2, G[alpha]q mRNA, and their proteins in severe acute pancreatitis (SAP) and BN52021 effects. METHODS Rats were assigned into negative-controlled group (NC), SAP-modeled group (SAP), and BN52051-remedial group (BN). Each group was assigned into 6 subgroups at different time points. G[alpha]i2, G[alpha]q mRNA, and their proteins were determined. RESULTS In the SAP group, G[alpha]i2 at 12 and 24 hours and G[alpha]q at 1 and 6 hours were remarkably higher than those in the NC group; in the BN Group, G[alpha]i2 is not remarkably different from that in the SAP group, but G[alpha]q at 1 and 6 hours was lower than those in the SAP group (P < 0.01), and G[alpha]i2 at 12 hours was higher than that in the NC group (P < 0.05), but G[alpha]q was not remarkably different from that in the NC group; in the SAP group, G[alpha]i2 and G[alpha]q proteins were higher than those in the NC group (P < 0.05); in the BN group, G[alpha]i2 proteins at 6, 12, and 24 hours and G[alpha]q proteins were lower than those in the SAP group (P < 0.05), and G[alpha]i2 and G[alpha]q proteins at each time phase point except 24 hours were higher than those in the NC group (P < 0.05). CONCLUSIONS G[alpha]i2, G[alpha]q mRNA, and their proteins in SAP increase. BN52021 decreases G[alpha]i2 and G[alpha]q. |
| Databáze: | OpenAIRE |
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