Lipid rafts sense and direct electric field-induced migration
| Autor: | Bo-jian Lin, Ling Chao, Pen-Hsiu Grace Chao, Shu-Kai Hu, Alexander B. Chen, Shun-hao Tsao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Integrins Swine Caveolin 1 Integrin Cell Culture Techniques Biology Mechanotransduction Cellular 03 medical and health sciences Membrane Microdomains Electricity Cell Movement Cell Line Tumor Caveolin Animals Humans Mechanotransduction Lipid raft Wound Healing Multidisciplinary Cell migration Raft Biological Sciences Cell biology 030104 developmental biology Membrane protein biology.protein Intracellular Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences. 114:8568-8573 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Endogenous electric fields (EFs) are involved in developmental regulation and wound healing. Although the phenomenon is known for more than a century, it is not clear how cells perceive the external EF. Membrane proteins, responding to electrophoretic and electroosmotic forces, have long been proposed as the sensing molecules. However, specific charge modification of surface proteins did not change cell migration motility nor directionality in EFs. Moreover, symmetric alternating current (AC) EF directs cell migration in a frequency-dependent manner. Due to their charge and ability to coalesce, glycolipids are therefore the likely primary EF sensor driving polarization of membrane proteins and intracellular signaling. We demonstrate that detergent-resistant membrane nanodomains, also known as lipid rafts, are the primary response element in EF sensing. The clustering and activation of caveolin and signaling proteins further stabilize raft structure and feed-forward downstream signaling events, such as rho and PI3K activation. Theoretical modeling supports the experimental results and predicts AC frequency-dependent cell and raft migration. Our results establish a fundamental mechanism for cell electrosensing and provide a role in lipid raft mechanotransduction. |
| Databáze: | OpenAIRE |
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