Reaction temperature alters chorzoxazone metabolism in carp (Cyprinus carpio) hepatic microsomes
| Autor: | Weiyuan Zhang, Jing Li, He Bin, Chunmei Wang, Lu Zheng, Hui Jiang, Zaijian Li, Shasha Wang, Jiyue Cao, Xiaocong Lu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Carps Physiology Aquatic Science Biochemistry Michaelis–Menten kinetics Cyprinus Cytochrome P-450 Enzyme System Pharmacokinetics Internal medicine medicine Animals Enzyme kinetics Carp chemistry.chemical_classification Chromatography biology Temperature General Medicine Metabolism biology.organism_classification Kinetics Chlorzoxazone Enzyme Endocrinology chemistry Microsomes Liver Drug metabolism |
| Zdroj: | Fish Physiology and Biochemistry. 38:1225-1231 |
| ISSN: | 1573-5168 0920-1742 |
| DOI: | 10.1007/s10695-012-9605-5 |
| Popis: | The study aimed to study the influence of different reaction temperatures on the carp (Cyprinus carpio) hepatic CYPs activity. Six groups of carp hepatic microsomes were incubated with probe drug (chorzoxazone) at 5, 10, 15, 20, 25 and 30°C separately. According to the principle of enzyme kinetics theory, the Michaelis constant (K (m)) value and maximum reaction velocity (V (max)) of CYPs (with CZX as probe) were obtained. The CYPs activity at different reaction temperatures was compared. In results, the K (m) values were separately 44.62, 31.35, 26.59, 21.75, 16.39, 29.69 μM, and the V (max) were separately 0.231, 0.234, 0.265, 0.294, 0.315, 0.239 nmol min(-1)mg(-1) at 5, 10, 15, 20, 25, 30°C. Results indicated that the enzyme CYPs activity was much higher at 25°C. It was also demonstrated that reaction temperature could affect the CYPs activity significantly. Therefore, this experiment builded a theoretical basis for the variations of fish pharmacokinetic parameters at different water temperatures and contributed to further research on the influence of water temperature on fish drug metabolism. |
| Databáze: | OpenAIRE |
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