Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system
| Autor: | Neil F. Fairweather, Steven N. Chatfield, B C Rodgers, A. J. Makoff, Ian G. Charles, Debbie E. Slater |
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| Rok vydání: | 1991 |
| Předmět: |
Baculoviridae
Clostridium tetani Protein Conformation medicine.drug_class viruses Genetic Vectors Molecular Sequence Data Immunology Moths Spodoptera medicine.disease_cause Monoclonal antibody Microbiology law.invention Mice Tetanus Toxin Affinity chromatography law Gangliosides parasitic diseases medicine Polyhedrin Animals Cloning Molecular Mice Inbred BALB C Base Sequence biology Toxin fungi biology.organism_classification Molecular biology Peptide Fragments Recombinant Proteins Infectious Diseases Recombinant DNA Parasitology Research Article |
| Zdroj: | Infection and Immunity. 59:1627-1632 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.59.5.1627-1632.1991 |
| Popis: | The baculovirus expression vector p36C was used to express in cells of the insect Spodoptera frugiperda fragment C of tetanus toxin under the control of the strong polyhedrin promoter. Fragment C was expressed intracellularly at a high level and was soluble, allowing it to be purified by affinity chromatography with monoclonal antibody TT08. Purified fragment C from baculovirus was used to immunize mice and was shown to successfully prevent the symptoms of tetanus following a toxin challenge. The ganglioside-binding properties of baculovirus-derived fragment C were compared with those of intact tetanus toxin and native fragment C and were found to be dissimilar. |
| Databáze: | OpenAIRE |
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