Comparison between 102k and 20k Poly(ethylene oxide) Depletants in Osmotic Pressure Measurements of Interfilament Forces in Cytoskeletal Systems
| Autor: | Stuart C. Feinstein, Cyrus R. Safinya, Joanna Deek, Youli Li, Peter J. Chung, Herbert P. Miller, Leslie Wilson, Myung Chul Choi, Chaeyeon Song |
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| Rok vydání: | 2022 |
| Předmět: |
0301 basic medicine
Materials science Polymers and Plastics Ethylene oxide Organic Chemistry Oxide 02 engineering and technology 021001 nanoscience & nanotechnology Polyelectrolyte Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Microtubule Materials Chemistry Biophysics Osmotic pressure 0210 nano-technology Cytoskeleton Poly ethylene |
| Zdroj: | ACS macro letters. 7(2) |
| ISSN: | 2161-1653 |
| Popis: | The proliferation of successful, cell-free reconstitutions of cytoskeletal networks have prompted measurements of forces between network elements via induced osmotic pressure by the addition of depletants. Indeed, it was through osmotic pressurization that Tau, an intrinsically disordered protein found in neuronal axons, was recently discovered to mediate two distinct microtubule (MT) bundle states, one widely spaced and a second tightly packed, separated by an energy barrier due to polyelectrolyte repulsions between opposing Tau projection domains on neighboring MT surfaces. Here, we compare interfilament force measurements in Tau coated MT bundles using PEO20k (poly(ethylene oxide), Mw = 20000), a commonly used inert depletant, and recently published measurements with PEO102k. While force measurements with either depletant reveals the transition between the two bundled states, measurements with PEO20k cannot recapitulate the correct critical pressure (Pc) at which widely spaced MT bundles transition to ... |
| Databáze: | OpenAIRE |
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