Hemodialysis Related Interleukin-2 Receptor Release by Peripheral Blood Mononuclear Cells
| Autor: | Bruno Memoli, Carmelo Libetta, A. Capuano, Luca De Nicola, Teresa Rampino, Vittorio E. Andreucci, Brunella Guida |
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| Přispěvatelé: | Memoli, B, Libetta, C, DE NICOLA, Luca, Rampino, T, Capuano, A, Guida, B, Andreucci, Ve, Memoli, Bruno, De Nicola, L, Capuano, Alfredo, Guida, Bruna |
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Interleukin 2 medicine.medical_specialty medicine.medical_treatment T cell Biomedical Engineering Biophysics Renal function Bioengineering In Vitro Techniques Peripheral blood mononuclear cell Blood cell Biomaterials Renal Dialysis Internal medicine Blood plasma medicine Humans Cellulose Receptor Uremia business.industry Receptors Interleukin-2 General Medicine Middle Aged medicine.anatomical_structure Endocrinology Cytokine Solubility Case-Control Studies Immunology Leukocytes Mononuclear Female business Kidneys Artificial medicine.drug |
| Zdroj: | ASAIO Journal. 42:60-63 |
| ISSN: | 1058-2916 |
| DOI: | 10.1097/00002480-199642010-00014 |
| Popis: | To evaluate the effects of hemodialysis treatment on the spontaneous cell release of interleukin-2 receptor (IL-2R), we studied 19 hemodialyzed patients (HD), 9 non hemodialyzed patients with chronic uremia (UR, glomerular filtration rate: 8.4 +/- 1.8 ml/min), and 8 healthy control subjects (C). We measured the release of IL-2R in the supernatant of peripheral blood mononuclear cells (PBMC) cultured for 24 hrs in Iscove's medium as well as the plasma levels of IL-2R. A significant increase of IL-2R release was detected in the supernatant of PBMC harvested from HD patients (32.4 +/- 2.4 and 34.2 +/- 5.6 U/3 x 10(6) PBMC daily before and after HD, respectively) as compared with UR (16.6 +/- 5.2 U/3 x 10(6) PBMC daily) and C (21.4 +/- 3.8 U/3 x 10(6) PBMC daily). Similarly, IL-2R plasma levels were significantly higher in HD (378.5 +/- 164.6 U/ml) than in UR (189.5 +/- 89.3 U/ml) and C (11.2 +/- 2.68 U/ml). To summarize, the current study demonstrates: a) an enhancement of spontaneous IL-2R cell release in HD patients; b) an increase of sIL-2R plasma levels in UR patients possibly related to reduced metabolism and/or urinary excretion, because it was not associated with high IL-2R cell release; and c) a further increment of IL-2R systemic levels in HD likely secondary to the high cell release of IL-2R. Therefore, a chronic T cell activation with increased release of IL-2R secondary to the dialysis procedure is suggested. |
| Databáze: | OpenAIRE |
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