Metallohelix vectors for efficient gene delivery via cationic DNA nanoparticles
| Autor: | Jaroslav Malina, Hana Kostrhunova, Vojtech Novohradsky, Peter Scott, Viktor Brabec |
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| Rok vydání: | 2022 |
| Předmět: |
TP
Molecular Structure Cell Survival AcademicSubjects/SCI00010 Genetic Vectors Gene Transfer Techniques Fluorescent Antibody Technique Gene Expression Metal Nanoparticles DNA Flow Cytometry Microscopy Atomic Force Transfection Cell Line Chemical Biology and Nucleic Acid Chemistry TA Genes Reporter Cations Genetics Humans Ferrous Compounds |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The design of efficient and safe gene delivery vehicles remains a major challenge for the application of gene therapy. Of the many reported gene delivery systems, metal complexes with high affinity for nucleic acids are emerging as an attractive option. We have discovered that certain metallohelices—optically pure, self-assembling triple-stranded arrays of fully encapsulated Fe—act as nonviral DNA delivery vectors capable of mediating efficient gene transfection. They induce formation of globular DNA particles which protect the DNA from degradation by various restriction endonucleases, are of suitable size and electrostatic potential for efficient membrane transport and are successfully processed by cells. The activity is highly structure-dependent—compact and shorter metallohelix enantiomers are far less efficient than less compact and longer enantiomers. Graphical Abstract Graphical AbstractCertain metallohelices—optically pure, selfcomplementing triple-stranded arrays of fully encapsulated Fe—are potent DNA condensing agents that act as nonviral DNA delivery vectors capable of mediating efficient gene transfection. |
| Databáze: | OpenAIRE |
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