Repeat RNAs associate with replication forks and post-replicative DNA
| Autor: | Cristina González-Aguilera, Anders H. Lund, Anja Groth, Helene M. Gylling, Martin A. Smith, Dominik C. Kaczorowski |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Biología Celular, Novo Nordisk Foundation, Danish Cancer Society. Denmark, European Research Council (ERC), Danish Council for Independent Research. Denmark, Lundbeck Foundation |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Biology
DNA replication Article Histones 03 medical and health sciences chemistry.chemical_compound Cell Line Tumor Humans Epigenetics Molecular Biology 030304 developmental biology 0303 health sciences 030302 biochemistry & molecular biology Cell Cycle RNA Epigenome DNA Repeats Non-coding RNA Chromatin Cell biology Histone chemistry biology.protein Protein Processing Post-Translational HeLa Cells |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname RNA Gylling, H M, Gonzalez-Aguilera, C, Smith, M A, Kaczorowski, D C, Groth, A & Lund, A H 2020, ' Repeat RNAs associate with replication forks and post-replicative DNA ', R N A, vol. 26, no. 9, pp. 1104-1117 . https://doi.org/10.1261/rna.074757.120 |
| Popis: | Noncoding RNA has a proven ability to direct and regulate chromatin modifications by acting as scaffolds between DNA and histone-modifying complexes. However, it is unknown if ncRNA plays any role inDNAreplication and epigenome maintenance, including histone eviction and reinstallment of histone modifications after genome duplication. Isolation of nascent chromatin has identified a large number of RNA-binding proteins in addition to unknown components of the replication and epigenetic maintenance machinery. Here, we isolated and characterized long and short RNAs associated with nascent chromatin at active replication forks and track RNA composition during chromatin maturation across the cell cycle. Shortly after fork passage, GA-rich-, alpha- and TElomeric Repeat-containing RNAs (TERRA) are associated with replicated DNA. These repeat containing RNAs arise from loci undergoing replication, suggesting an interaction in cis. Postreplication during chromatin maturation, and even after mitosis in G1, the repeats remain enriched on DNA. This suggests that specific types of repeat RNAs are transcribed shortly after DNA replication and stably associate with their loci of origin throughout the cell cycle. The presented method and data enable studies of RNA interactions with replication forks and post-replicative chromatin and provide insights into how repeat RNAs and their engagement with chromatin are regulated with respect to DNA replication and across the cell cycle. |
| Databáze: | OpenAIRE |
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