Thrombin-Activated Human Endothelial Cells Support Monocyte Adhesion In Vitro Following Expression of Intercellular Adhesion Molecule-1 (ICAM-1; CD54) and Vascular Cell Adhesion Molecule-1 (VCAM-1; CD106)
| Autor: | Catherine Farnarier, Martine Fabrigoule, Pierre Bongrand, Anne-Marie Benoliel, Gilles Kaplanski, Valérie Marin, Vera Boulay, S. Kaplanski |
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| Rok vydání: | 1998 |
| Předmět: |
Umbilical Veins
Sialoglycoproteins Immunology Intercellular Adhesion Molecule-1 Hirudin Vascular Cell Adhesion Molecule-1 Biology Biochemistry Monocytes chemistry.chemical_compound Thrombin Cell–cell interaction Cell Adhesion medicine Humans RNA Messenger VCAM-1 Cell adhesion Tumor Necrosis Factor-alpha Cell adhesion molecule Antibodies Monoclonal Cell Biology Hematology Hirudins Peptide Fragments Cell biology Interleukin 1 Receptor Antagonist Protein Gene Expression Regulation chemistry Leukocytes Mononuclear Receptors Thrombin Endothelium Vascular Selectin medicine.drug |
| Zdroj: | ResearcherID |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Thrombin, a central molecule in coagulation, is also involved in inflammation. Notably, thrombin induces endothelial neutrophil adhesion, P- and E-selectin expression, and chemokine production. We show here that thrombin induces expression of intercellular adhesion molecule-1 (ICAM-1; CD54) and vascular cell adhesion molecule-1 (VCAM-1; CD106) on human umbilical vein endothelial cells (HUVECs) associated with increased adhesion of monocytes. Thrombin increased mRNA steady-state levels and expression of ICAM-1 over 24 hours. Thrombin-induced VCAM-1 expression exhibited unusual kinetics, reaching maximum levels after 6 to 12 hours, but decreasing to near baseline after 24 hours. Thrombin activity on HUVECs was mediated through interaction with its specific receptor, because ICAM-1 and VCAM-1 expression were similarly induced by the 14-amino acid thrombin receptor-activating peptide. Thrombin-induced ICAM-1 and VCAM-1 expression was significantly inhibited by hirudin, but not by interleukin-1 receptor antagonist or anti-tumor necrosis factor monoclonal antibody (MoAb). Thrombin-activated HUVECs significantly increased greater numbers of adhering THP-1 macrophagic cells, peripheral blood mononuclear cells, or purified monocytes than unstimulated HUVECs. This adhesion was inhibited by anti-CD18 and anti-CD49d MoAb, demonstrating that thrombin-induced ICAM-1 and VCAM-1 were functional. These results show that, in addition to selectins, thrombin directly induces a cytokine-independent expression of adhesion molecules of the Ig superfamily on HUVECs that may support firm leukocyte attachment during inflammation.© 1998 by The American Society of Hematology. |
| Databáze: | OpenAIRE |
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