Photodynamic inactivation of prions by disulfonated hydroxyaluminium phthalocyanine
| Autor: | Karel Holada, Jan Rakusan, Olga Janouskova, Marie Karaskova |
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| Rok vydání: | 2012 |
| Předmět: |
Indoles
Time Factors Prions chemistry.chemical_element Scrapie Isoindoles Biology Oxygen Cell Line Mice chemistry.chemical_compound Virology Pressure Animals Thermolabile Neurons chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug Molecular Structure Singlet oxygen Temperature Brain Combinatorial chemistry Yeast In vitro Photochemotherapy chemistry Biochemistry Nucleic acid Sulfonic Acids |
| Zdroj: | Journal of General Virology. 93:2512-2517 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.044727-0 |
| Popis: | Sulfonated phthalocyanines (Pcs) are cyclic tetrapyrroles that constitute a group of photosensitizers. In the presence of visible light and diatomic oxygen, Pcs produce singlet oxygen and other reactive oxygen species that have known degradation effects on lipids, proteins and/or nucleic acids. Pcs have been used successfully in the treatment of bacterial, yeast and fungal infections, but their use in the photodynamic inactivation of prions has never been reported. Here, we evaluated the photodynamic activity of the disodium salt of disulfonated hydroxyaluminium phthalocyanine (PcDS) against mouse-adapted scrapie RML prions in vitro. PcDS treatment of RML brain homogenate resulted in a time- and dose-dependent inactivation of prions. The photodynamic potential of Pcs offers a new way to inactivate prions using biodegradable compounds at room temperature and normal pressure, which could be useful for treating thermolabile materials and liquids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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