Optimization strategies for the analysis and purification of drug discovery compounds by reversed-phase high-performance liquid chromatography with high-pH mobile phases
| Autor: | Cristina Anta, Alfonso Espada, Aránzazu Marín |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Chromatography
Resolution (mass spectrometry) Chemistry Drug discovery Elution Organic Chemistry General Medicine Reversed-phase chromatography Hydrogen-Ion Concentration Mass spectrometry Biochemistry Mass loading High-performance liquid chromatography Analytical Chemistry Phase (matter) Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 1030:43-51 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2003.12.027 |
| Popis: | Careful selection of both high-pH mobile phase as well as organic modifier, was performed in order to develop and optimize HPLC conditions for the separation of drug discovery compounds. High-pH mobile phases provide excellent chromatographic resolution and increased mass loading of basic compounds. The analytical methods so defined have been successfully transferred to preparative automated UV-directed purification, an important fact due to the increasing number of samples requiring purification. It should be noted that, the single prerequisite for this approach is an analytical LC-UV-MS run, therefore the system has the ability to collect only fractions likely to contain the target product. A cost-effective strategy for maximizing the purification of drug discovery compounds is proposed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect