Mapping and Cloning of Late Blight Resistance Genes from Solanum venturii Using an Interspecific Candidate Gene Approach

Autor: Herman J. van Eck, Tae-Ho Park, Evert Jacobsen, Hendrik Rietman, Jonathan D. G. Jones, S. J. Foster, Gert van Arkel, Edwin A. G. van der Vossen, Richard G. F. Visser, Mathieu A. Pel
Jazyk: angličtina
Rok vydání: 2009
Předmět:
DNA
Plant
Physiology
Sequence analysis
Phytophthora infestans
phytophthora-infestans
Molecular Sequence Data
Plant disease resistance
Solanum
Chromosomes
Plant
nicotiana-benthamiana
quantitative trait locus
Laboratorium voor Plantenveredeling
Gene mapping
r-gene
PRI Biodiversiteit en Veredeling
Tobacco
Tomato mosaic virus
Amino Acid Sequence
Cloning
Molecular
field-resistance
Gene
lycopersicon-esculentum
Plant Diseases
Plant Proteins
Genetics
Oomycete
biology
Sequence Homology
Amino Acid
EPS-2
nucleotide-binding
Genetic Complementation Test
fungi
nbs-lrr proteins
Chromosome Mapping
food and beverages
General Medicine
R gene
Sequence Analysis
DNA
biology.organism_classification
Plants
Genetically Modified
Immunity
Innate
Plant Leaves
PRI Biodiversity and Breeding
Plant Breeding
disease-resistance
Host-Pathogen Interactions
Agronomy and Crop Science
race-specific resistance
Zdroj: Molecular Plant-Microbe Interactions, 22(5), 601-615
Molecular Plant-Microbe Interactions 22 (2009) 5
ISSN: 0894-0282
Popis: Late blight, caused by the oomycete Phytophthora infestans, is one of the most devastating diseases of potato. Resistance (R) genes from the wild species Solanum demissum have been used by breeders to generate late-blight-resistant cultivars but resistance was soon overcome by the pathogen. A more recent screening of a large number of wild species has led to the identification of novel sources of resistance, many of which are currently being characterized further. Here, we report on the cloning of dominant Rpi genes from S. venturii. Rpi-vnt1.1 and Rpi-vnt1.3 were mapped to chromosome 9 using nucleotide binding site (NBS) profiling. Subsequently, a Tm-22-based allele mining strategy was used to clone both genes. Rpi-vnt1.1 and Rpi-vnt1.3 belong to the coiled-coil NBS leucine-rich repeat (LRR) class of plant R genes and encode predicted peptides of 891 and 905 amino acids (aa), respectively, which share 75% amino acid identity with the Tomato mosaic virus resistance protein Tm-22 from tomato. Compared with Rpi-vnt1.1, Rpi-vnt1.3 harbors a 14-aa insertion in the N-terminal region of the protein and two different amino acids in the LRR domain. Despite these differences, Rpi-vnt1.1 and Rpi-vnt1.3 genes have the same resistance spectrum.
Databáze: OpenAIRE
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