Heterogeneity Among Ly-49C Natural Killer (NK) Cells: Characterization of Highly Related Receptors with Differing Functions and Expression Patterns
| Autor: | Dixie L. Mager, Fumio Takei, Freeman Jd, S Lemieux, Jack Brennan |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Molecular Sequence Data
Immunology Biology Transfection Polymerase Chain Reaction Article CD49b Mice Species Specificity Antigen Complementary DNA Animals Antigens Ly Immunology and Allergy Lectins C-Type Amino Acid Sequence Receptor Peptide sequence DNA Primers Mice Inbred BALB C Membrane Glycoproteins COS cells Base Sequence Sequence Homology Amino Acid Cell Membrane Articles Flow Cytometry Molecular biology Recombinant Proteins Killer Cells Natural Mice Inbred C57BL COS Cells biology.protein Antibody Receptors NK Cell Lectin-Like |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| DOI: | 10.1084/jem.184.6.2085 |
| Popis: | Ly-49C is a member of the polymorphic family of murine NK cell inhibitory receptors. The 5E6 antibody that defines a subset of NK cells responsible for the rejection of parental H-2d bone marrow by F1 mice has been shown previously to react with Ly-49C. Here, the 5E6 antibody was found to detect two Ly-49C-related molecules in B6 mice. Two cDNA clones were isolated from B6 NK cells, one identical to previously reported Ly-49CB6 and the other a novel cDNA. The deduced amino acid sequence of the latter differs from that of Ly-49CBALB at only 4 residues, whereas the previously reported Ly-49CB6 differs at 22 residues. Flow cytometric analyses of COS cells transfected with the two cDNAs showed that the 5E6 antibody binds to both Ly-49 molecules, while another anti-Ly-49C antibody, 4LO3311, binds to the newly described Ly-49C but not the previously reported Ly-49CB6. Two-color flow cytometric analysis detected 5E6+4LO3311− as well as 5E6+4LO3311+ subsets of NK cells from B6, but not BALB/c, mice. The level of Ly-49C expression on B6 NK cells detected by the 4LO3311 antibody was substantially lower than that on BALB/c NK cells. Binding specificity of the novel Ly-49CB6 was indistinguishable from that of Ly-49CBALB, whereas no binding was detectable with previously reported Ly-49CB6. These results demonstrate that the newly described Ly-49CB6, not the previously reported Ly-49CB6, is the probable B6 allelic form of Ly49C. The previously reported Ly-49CB6 must be encoded by a separate gene and should be renamed Ly-49I. The implication of these results with respect to the role of Ly-49C in hybrid resistance is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|