Identification and mapping of RAPD and RFLP markers linked to a fertility restorer gene for a new source of cytoplasmic male sterility in Beta vulgaris ssp. maritima
| Autor: | Philippe Vernet, Didier Merdinoglu, Gisèle Butterlin, Joël Cuguen, V. Laporte, Pierre Saumitou-Laprade |
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| Přispěvatelé: | Génétique et évolution des populations végétales (GEPV), Université de Lille, Sciences et Technologies-Centre National de la Recherche Scientifique (CNRS), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, ProdInra, Migration |
| Rok vydání: | 1998 |
| Předmět: |
[SDE] Environmental Sciences
0106 biological sciences Nuclear gene [SDV]Life Sciences [q-bio] Locus (genetics) Biology 01 natural sciences 03 medical and health sciences Gene mapping RAPD Genetic linkage Genetics ComputingMilieux_MISCELLANEOUS health care economics and organizations 030304 developmental biology 0303 health sciences Cytoplasmic male sterility General Medicine [SDV] Life Sciences [q-bio] Genetic marker [SDE]Environmental Sciences [SDE.BE]Environmental Sciences/Biodiversity and Ecology Restriction fragment length polymorphism Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | TAG Theoretical and Applied Genetics TAG Theoretical and Applied Genetics, Springer Verlag, 1998, 96 (6-7), pp.989-996. ⟨10.1007/s001220050830⟩ TAG Theoretical and Applied Genetics, Springer Verlag, 1998, 96, pp.989-996 |
| ISSN: | 1432-2242 0040-5752 |
| DOI: | 10.1007/s001220050830 |
| Popis: | The present study shows that the recently described mitochondrial H haplotype is associated with cytoplasmic male-sterility (CMS). This new source of CMS appears to be di⁄erent from the mitotype E-associated CMS most frequently found in natural populations. A mitotype H progeny with a sexual phenotype segregation was used to identify a gene restoring male fertility (R1H ). Using bulk segregant analysis (BSA), nine RAPD markers linked to this restorer locus were detected and mapped. The compari- son with other Beta genetic maps shows that the closest RAPD marker, distant from R1H by 5.2 cM, belongs to the same linkage group as the monogermy locus. In order to determine the position of R1H more precisely, four RFLP loci within this linkage group were mapped in the segregating progeny. It thus became possible to construct a linkage map of the region containing the RFLP, RAPD and R1H loci. The closest RFLP marker was located 1.7 cM away from R1H. However, a nuclear gene restoring the 'Owen' CMS which is currently used in sugar beet breeding is reportedly linked to the mono- germy locus, raising the question of a possible identity between the new CMS system and the 'Owen' CMS. |
| Databáze: | OpenAIRE |
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