The effect of catecholamines and adenosine deaminase on the glucose transport system in rat adipocytes
| Autor: | Thomas Røll Larsen, Bowes Sb, Jørgen Gliemann, William D. Rees |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
medicine.medical_specialty Adenosine Epinephrine Adenosine Deaminase Biological Transport Active Nucleoside Deaminases Deoxyglucose In Vitro Techniques Biology chemistry.chemical_compound Adenosine Triphosphate Adenosine deaminase Adipocyte Internal medicine medicine Animals Insulin Molecular Biology Hexose transport Epididymis HEPES Glucose transporter Methylglucosides Rats Inbred Strains AMP deaminase Cell Biology Rats Glucose Endocrinology Adipose Tissue chemistry Biochemistry biology.protein Catecholamine 3-O-Methylglucose medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 845:373-379 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/0167-4889(85)90201-0 |
| Popis: | 2-Deoxyglucose uptake (3 min) and 3-O- methylglucose transport (2 s) was measured in rat adipocytes preincubated with 5 μM epinephrine plus adenosine deaminase as described by Green (Green, A. (1983) FEBS Lett. 152, 261–264). 2-Deoxyglucose uptake was about 95% depressed in insulin-treated, but not in ‘basal’, cells preincubated with epinephrine plus adenosine deaminase for 60 min in broad agreement with Green's report. However, this depression was caused by a decrease in sugar phosphorylation rather than transport. In similarly incubated cells, transport of 3-O- methylglucose , a sugar analogue not phosphorylated in the adipocytes, was not affected by catecholamine plus adenosine deaminase. However, a decrease in transport of about 60% was observed both in the absence and the presence of insulin when the albumin concentration was high enough and the cell concentration low enough to prevent accumulation of free fatty acids in the medium. In addition, the insulin sensitivity with regard to hexose transport was markedly reduced. Transport was approximately doubled in cells incubated with 5 μM epinephrine in the absence of adenosine deaminase. Thus, epineprine at a high concentration stimulates hexose transport in the absence of adenosine deaminase (presence of adenosine) whereas it inhibits both basal and insulin-stimulated transport in the presence of adenosine deaminase (absence of adenosine). |
| Databáze: | OpenAIRE |
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