Abnormal DNA methylation patterns in patients with infection‑caused leukocytopenia based on methylation microarrays
| Autor: | Dan Luo, Yafang Li, Xiaohong Yang, Wenyi Wang, Mingqin Deng, Rong Jin, Chao Wu, Zhichuang Lian, Xirennayi Muhataer |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Cancer Research Leukocytosis Ubiquitin-Protein Ligases leukocytopenia Severity of Illness Index Biochemistry Leukocyte Count Leukocytopenia gene chip technology Genetics medicine Humans Molecular Biology Aged Oligonucleotide Array Sequence Analysis Regulation of gene expression business.industry bacterial infection Case-control study Complement C5 Articles Complement C3 Leukopenia Methylation DNA Methylation Middle Aged Molecular biology Molecular medicine C-Reactive Protein Gene Ontology Oncology CpG site Case-Control Studies DNA methylation Molecular Medicine CpG Islands Female methylation medicine.symptom business WW domain containing E3 ubiquitin protein ligase 2 Procalcitonin |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2020.11061 |
| Popis: | The present study aimed to investigate the association between gene methylation and leukocytopenia from the perspective of gene regulation. A total of 30 patients confirmed as having post-infection leukocytopenia at People's Hospital of Xinjiang Uygur Autonomous Region between January 2016 and June 2017 were successively recruited as the leukocytopenia group; 30 patients with post-infection leukocytosis were enrolled as the leukocytosis group. In addition, 30 healthy volunteers who received a health examination at the hospital during the same period were included as the normal control group. In each group, four individuals were randomly selected for whole genome methylation screening. After selection of key methylation sites, the remaining samples in each group were used for verification using matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The levels of serum complement factors C3 and C5 in the leukocytopenia group were significantly lower than those in the other two groups (P |
| Databáze: | OpenAIRE |
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