Genotypic Variability of HIV-1 Reverse Transcriptase Gene from Long-Term Antiretroviral-Experienced Patients in Kenya
| Autor: | Anne W. T. Muigai, Bernhards Ogutu, Timothy J. Nzomo, Javan Okendo, Raphael Lwembe, Geoffrey Masankwa, Maureen J. Kimulwo, Rose C. Kitawi, Ruth S. Mwatelah, Rashid Aman, Washingtone Ochieng |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Adolescent Genotype Genotyping Techniques Immunology Molecular Sequence Data HIV Infections Biology Young Adult Phylogenetics Virology Genetic variation Humans Young adult Gene Phylogeny Phylogenetic tree Genetic Variation Sequence Notes Sequence Analysis DNA Middle Aged Kenya Reverse transcriptase Subtyping HIV Reverse Transcriptase Infectious Diseases Anti-Retroviral Agents HIV-1 Female |
| Popis: | There is continuous need to track genetic profiles of HIV strains circulating in different geographic settings to hasten vaccine discovery and inform public health and intervention policies. We partially sequenced the reverse transcriptase region of the HIV-1 pol gene from a total of 54 Kenyan patients aged 18–56 years who continued highly active antiretroviral treatment (HAART) for between 8 and 102 months. Subtyping was done using both the JPHMM tool and phylogenetic method. HIV-1 subtype A1 was the predominant strain in circulation, representing 57.4% and 70.4% of all isolates as determined by JPHMM and phylogenetic methods, respectively. Subtypes D (14.8%, 7.4%), C (5.6%, 9.3%), and A2 (0%, 5.6%) were determined at respective prevalence by both methods. JPHMM identified 22.2% of the isolates as recombinants. This surveillance focused on the RT gene and reaffirms the predominance of subtype A and an increasing proportion of recombinant strains in the Kenyan epidemic. |
| Databáze: | OpenAIRE |
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